Bryon Donohoe

2024

Squeezed Light Stimulated Raman Scattering (SRS) Microscopy for Bioenergy Applications

Zeng, Y., Yi, Z., Agarwal, G., Scully, M. & Donohoe, B., 2024, 17 p.

Research output: NREL › Presentation

2023

Amino Acids Essential for the Assembly of Cellulose Synthase Complexes

Olek, A., Rushton, P., Kihara, D., Aryal, U., Ciesielski, P., Donohoe, B., Zhang, Z., Stauffacher, C., McCann, M. & Carpita, N., 2023, 13 p.

Research output: NREL › Presentation

Feedstock Variability: Causes, Consequences and Mitigation of Biological Degradation

Donohoe, B., Wendt, L., Ding, L. & Smith, W., 2023, In: Biomass Magazine. 16, 1, p. 34-36 3 p.

Research output: Contribution to journal › Article

Interlaboratory Study: Testing Reproducibility of Solid Biofuels Component Identification Using Reflected Light Microscopy: Article No. 104331

Drobniak, A., Mastalerz, M., Jelonek, Z., Jelonek, I., Adsul, T., Andolsek, N., Ardakani, O., Congo, T., Demberelsuren, B., Donohoe, B., Douds, A., Flores, D., Ganzorig, R., Ghosh, S., Gize, A., Goncalves, P., Hackley, P., Hatcherian, J., Hower, J. & Kalaitzidis, S. & 26 others, Kedzior, S., Knowles, W., Kus, J., Lis, K., Lis, G., Liu, B., Luo, Q., Du, M., Mishra, D., Misz-Kennan, M., Mugerwa, T., Nedzweckas, J., O'Keefe, J., Park, J., Pearson, R., Petersen, H., Reyes, J., Ribeiro, J., de la Rosa-Rodriguez, G., Sosnowski, P., Valentine, B., Varma, A., Wojtaszek-Kalaitzidi, M., Xu, Z., Zdravkov, A. & Ziemianin, K., 2023, In: International Journal of Coal Geology. 277, 13 p.

Research output: Contribution to journal › Article › peer-review

3 Scopus Citations

2022

Image Analysis for Rapid Assessment and Quality-Based Sorting of Corn Stover

Ding, L., Hoover, A., Emerson, R., Lin, K.-T., Gruber, J., Donohoe, B., Klinger, J., Colby, R., Thomas, B., Smith, W. & Ray, A., 2022, In: Frontiers in Energy Research. 10, 15 p., 837698.

Research output: Contribution to journal › Article › peer-review

Open Access

2 Scopus Citations

Real-Time Biomass Feedstock Particle Quality Detection Using Image Analysis and Machine Vision

Gudavalli, C., Bose, E., Donohoe, B. & Sievers, D., 2022, In: Biomass Conversion and Biorefinery. 12, 12, p. 5739-5750 12 p.

Research output: Contribution to journal › Article › peer-review

Open Access

9 Scopus Citations

2021

Comparison of Methodologies Used to Determine Aromatic Lignin Unit Ratios in Lignocellulosic Biomass

Happs, R., Addison, B., Doeppke, C., Donohoe, B., Davis, M. & Harmon-Ware, A., 2021, In: Biotechnology for Biofuels. 14, 1, 16 p., 58.

Research output: Contribution to journal › Article › peer-review

Open Access

26 Scopus Citations

Distribution of Bound and Free Water in Anatomical Fractions of Pine Residues and Corn Stover as a Function of Biological Degradation

Ding, L., Gruber, J., Ray, A., Donohoe, B. & Li, C., 29 Nov 2021, In: ACS Sustainable Chemistry and Engineering. 9, 47, p. 15884-15896 13 p.

Research output: Contribution to journal › Article › peer-review

8 Scopus Citations

Flow Behavior Characterization of Biomass Feedstocks

Donohoe, B., Cheng, Z., Leal, J., Hartford, C., Carson, J., Craig, D., Xia, Y., Daniel, R., Ajayi, O. & Semelsberger, T., 2021, In: Powder Technology. 387, p. 156-180 25 p.

Research output: Contribution to journal › Article › peer-review

Open Access

28 Scopus Citations

Iron Incorporation Both Intra- and Extra-Cellularly Improves the Yield and Saccharification of Switchgrass (Panicum virgatum L.) Biomass

Lin, C.-Y., Donohoe, B., Bomble, Y., Yang, H., Yunes, M., Sarai, N., Shollenberger, T., Decker, S., Chen, X., McCann, M., Tucker, M., Wei, H. & Himmel, M., 2021, In: Biotechnology for Biofuels. 14, 1, 15 p., 55.

Research output: Contribution to journal › Article › peer-review

Open Access

4 Scopus Citations

2020

Chapter 6: An Improved Leaf Protoplast System for Highly Efficient Transient Expression in Switchgrass (Panicum virgatum L.)

Lin, C.-Y., Wei, H., Donohoe, B., Tucker, M. & Himmel, M., 2020, Metabolic Pathway Engineering: Methods in Molecular Biology, Volume 2096. Himmel, M. E. & Bomble, Y. J. (eds.). Humana Press Inc., p. 61-79 19 p. (Methods in Molecular Biology; vol. 2096).

Research output: Chapter in Book/Report/Conference proceeding › Chapter › peer-review

1 Scopus Citations

Characterization and Engineering of a Two-Enzyme System for Plastics Depolymerization

Knott, B., Erickson, E., Allen, M., Gado, J., Graham, R., Kearns, F., Pardo, I., Topuzlu, E., Anderson, J., Austin, H., Dominick, G., Johnson, C., Rorrer, N., Szostkiewicz, C., Copié, V., Payne, C., Woodcock, H., Donohoe, B., Beckham, G. & McGeehan, J., 13 Oct 2020, In: Proceedings of the National Academy of Sciences of the United States of America. 117, 41, p. 25476-25485 10 p.

Research output: Contribution to journal › Article › peer-review

Open Access

316 Scopus Citations

Characterizing Variability in Lignocellulosic Biomass: A Review

Yan, J., Oyedeji, O., Leal, J., Donohoe, B., Semelsberger, T., Li, C., Hoover, A., Webb, E., Bose, E., Zeng, Y., Williams, C. L., Schaller, K., Sun, N., Ray, A. & Tanjore, D., 8 Jun 2020, In: ACS Sustainable Chemistry and Engineering. 8, 22, p. 8059-8085 27 p.

Research output: Contribution to journal › Article › peer-review

63 Scopus Citations

Chemical and Structural Changes in Corn Stover After Ensiling: Influence on Bioconversion

Nagle, N., Donohoe, B., Wolfrum, E., Kuhn, E., Haas, T., Ray, A., Wendt, L., Delwiche, M., Weiss, N. & Radtke, C., 14 Aug 2020, In: Frontiers in Bioengineering and Biotechnology. 8, 14 p., 739.

Research output: Contribution to journal › Article › peer-review

Open Access

9 Scopus Citations

Ferrous and Ferric Ion-Facilitated Dilute Acid Pretreatment of Lignocellulosic Biomass under Anaerobic or Aerobic Conditions: Observations of Fe Valence Interchange and the Role of Fenton Reaction

Wei, H., Wang, W., Ciesielski, P., Donohoe, B., Zhang, M., Himmel, M., Chen, X. & Tucker, M., 2020, In: Molecules. 25, 6, 12 p., 1427.

Research output: Contribution to journal › Article › peer-review

Open Access

5 Scopus Citations

High Rate CO2 Valorization to Organics via CO Mediated Silica Nanoparticle Enhanced Fermentation: Article No. 115725

Jack, J., Lo, J., Donohoe, B., Maness, P.-C. & Ren, Z. J., 2020, In: Applied Energy. 279, 11 p.

Research output: Contribution to journal › Article › peer-review

7 Scopus Citations

Impacts of Biological Heating and Degradation during Bale Storage on the Surface Properties of Corn Stover

Bose, E., Leal, J., Hoover, A., Zeng, Y., Li, C., Ray, A., Semelsberger, T. & Donohoe, B., 21 Sep 2020, In: ACS Sustainable Chemistry and Engineering. 8, 37, p. 13973-13983 11 p.

Research output: Contribution to journal › Article › peer-review

6 Scopus Citations

Impacts of Inorganic Material (Total Ash) on Surface Energy, Wettability, and Cohesion of Corn Stover

Donohoe, B., Resch, M., Leal, J., Torres, E., Rouse, W., Moore, C., Sutton, A., Hoover, A., Li, C., Ray, A. & Semlsberger, T., 3 Feb 2020, In: ACS Sustainable Chemistry and Engineering. 8, 4, p. 2061-2072 12 p.

Research output: Contribution to journal › Article › peer-review

12 Scopus Citations

Insights into Mechanisms of Screw Feeder Plugging by Heated Pine Forestry Residues Using in-situ and Correlative Microscopy

Gruber, J., Zeng, Y., Rowland, S., Carpenter, D. & Donohoe, B., 2020

Research output: NREL › Poster

Leveraging Super High Optical Resolution Microscopy to Probe the Interaction Zone Between Clostridium thermocellum and Biomass

Yarbrough, J., Stich, D., Chung, D., Hengge, N., Vinzant, T., Huang, S., Zeng, Y., Donohoe, B., Himmel, M., Bomble, Y. & Tuskan, G., 2020

Research output: NREL › Poster

Mesoscale Reaction-Diffusion Phenomena Governing Lignin-First Biomass Fractionation

Thornburg, N., Pecha, M. B., Brandner, D., Reed, M., Vermaas, J., Michener, W., Katahira, R., Vinzant, T., Foust, T., Donohoe, B., Roman-Leshkov, Y., Ciesielski, P. & Beckham, G., 2020, In: ChemSusChem. 13, 17, p. 4495-4509 15 p.

Research output: Contribution to journal › Article › peer-review

43 Scopus Citations

Multiscale Characterization of Lignocellulosic Biomass Variability and Its Implications to Preprocessing and Conversion: A Case Study for Corn Stover

Donohoe, B., Wolfrum, E., Resch, M., Ray, A., Williams, C., Hoover, A., Li, C., Sale, K., Emerson, R., Klinger, J., Oksen, E., Narani, A., Yan, J., Beavers, C., Tanjore, D., Leal, J., Bowen, J., Semlsberger, T., Yunes, M. & Bose, E., 2 Mar 2020, In: ACS Sustainable Chemistry and Engineering. 8, 8, p. 3218-3230 13 p.

Research output: Contribution to journal › Article › peer-review

Open Access

31 Scopus Citations

Outer Membrane Vesicles Catabolize Lignin-Derived Aromatic Compounds in Pseudomonas putida KT2440

Salvachua, D., Werner, A., Pardo, I., Michalska, M., Black, B., Donohoe, B., Haugen, S., Katahira, R., Notonier, S., Ramirez, K., Amore, A., Purvine, S., Zink, E., Abraham, P., Giannone, R., Poudel, S., Laible, P., Hettich, R. & Beckham, G., 28 Apr 2020, In: Proceedings of the National Academy of Sciences of the United States of America. 117, 17, p. 9302-9310 9 p.

Research output: Contribution to journal › Article › peer-review

Open Access

90 Scopus Citations

Synthetic Fungal Multifunctional Cellulases for Enhanced Biomass Conversion

Brunecky, R., Subramanian, V., Yarbrough, J., Donohoe, B., Vinzant, T., VanderWall, T., Knott, B., Chaudhari, Y., Bomble, Y., Himmel, M. & Decker, S., 2020, In: Green Chemistry. 22, 2, p. 478-489 12 p.

Research output: Contribution to journal › Article › peer-review

Open Access

29 Scopus Citations

2019

Chemical and Structural Effects on the Rate of Xylan Hydrolysis during Dilute Acid Pretreatment of Poplar Wood

Mittal, A., Pilath, H., Parent, Y., Donohoe, B., Yarbrough, J., Black, S., Himmel, M., Nimlos, M., Johnson, D. & Chatterjee, S., 4 Mar 2019, In: ACS Sustainable Chemistry and Engineering. 7, 5, p. 4842-4850 9 p.

Research output: Contribution to journal › Article › peer-review

13 Scopus Citations

Exoproteomics Reveals Outer Membrane Vesicle Mediated Catabolism of Lignin-Derived Aromatics by P. putida KT2440

Werner, A., Salvachua Rodriguez, D., Donohoe, B., Beckham, G., Abraham, P., Giannone, R., Hettich, B., Michalska, M. & Laible, P., 2019

Research output: NREL › Poster

Overcoming Cellulose Recalcitrance in Woody Biomass for the Lignin-First Biorefinery

Donohoe, B., Yang, H., Zhang, X., Luo, H., Liu, B., Shiga, T., Li, X., Kim, J., Rubinelli, P., Overton, J., Subramanyam, V., Cooper, B., Mo, H., Abu-Omar, M., Chapple, C., Makowski, L., Mosier, N., McCann, M., Carpita, N. & Meilan, R., 2019, In: Biotechnology for Biofuels. 12, 1, 18 p., 171.

Research output: Contribution to journal › Article › peer-review

Open Access

44 Scopus Citations

2018

3D Electron Tomographic and Biochemical Analysis of ER, Golgi and trans Golgi Network Membrane Systems in Stimulated Venus Flytrap (Dionaea muscipula) Glandular Cells

Donohoe, B., Gergely, Z., Martinez, D., Mogelsvang, S., Herder, R. & Staehelin, L., 2018, In: Journal of Biological Research (Greece). 25, 1, 16 p., 15.

Research output: Contribution to journal › Article › peer-review

Open Access

11 Scopus Citations

An Iterative Computational Design Approach to Increase the Thermal Endurance of a Mesophilic Enzyme

Sammond, D., Donohoe, B., Alahuhta, P., Lunin, V., Chung, D., Mittal, A., Himmel, M., Bomble, Y., Kastelowitz, N., Yin, H., Guss, A. & Sarai, N., 2018, In: Biotechnology for Biofuels. 11, 1, 13 p., 189.

Research output: Contribution to journal › Article › peer-review

Open Access

10 Scopus Citations

Chapter 16: Lignin Visualization: Advanced Microscopy Techniques for Lignin Characterization

Zeng, Y. & Donohoe, B., 2018, Lignin Valorization: Emerging Approaches: RSC Energy and Environment Series, No. 19. Beckham, G. T. (ed.). 19 ed. Royal Society of Chemistry, p. 477-498 22 p. (RSC Energy and Environment Series; vol. 2018-January, no. 19).

Research output: Chapter in Book/Report/Conference proceeding › Chapter › peer-review

3 Scopus Citations

Characterization and Engineering of a Plastic Degrading Aromatic Polyesterase

Austin, H. P., Allen, M. D., Donohoe, B. S., Rorrer, N. A., Kearns, F. L., Silveira, R. L., Pollard, B. C., Dominick, G., Duman, R., Omari, K. E., Mykhaylyk, V., Wagner, A., Michener, W. E., Amore, A., Skaf, M. S., Crowley, M. F., Thorne, A. W., Johnson, C. W., Lee Woodcock, H. & McGeehan, J. E. & 1 others, Beckham, G. T., 2018, In: Proceedings of the National Academy of Sciences of the United States of America. 115, 19, p. E4350-E4357

Research output: Contribution to journal › Article › peer-review

Open Access

704 Scopus Citations

Fast Pyrolysis of Opuntia ficus-indica (Prickly Pear) and Grindelia squarrosa (Gumweed)

Mukarakate, C., Nimlos, M., Carpenter, D., Donohoe, B., Cross, P., Mayer, J., Cushman, J., Neupane, B., Miller, G. & Adhikari, S., 2018, In: Energy and Fuels. 32, 3, p. 3510-3518 9 p.

Research output: Contribution to journal › Article › peer-review

8 Scopus Citations

Oleaginicity of the Yeast Strain Saccharomyces cerevisiae D5A

Knoshaug, E., Donohoe, B., Van Wychen, S., Zhang, M., Himmel, M., Yang, S., Yang, Y. & He, Q., 2018, In: Biotechnology for Biofuels. 11, 1, 20 p., 258.

Research output: Contribution to journal › Article › peer-review

Open Access

36 Scopus Citations

Reassessing the Roles of PIN Proteins and Anticlinal Microtubules During Pavement Cell Morphogenesis

Donohoe, B., Belteton, S., Sawchuk, M., Scarpella, E. & Szymanski, D., 2018, In: Plant Physiology. 176, 1, p. 432-449 18 p.

Research output: Contribution to journal › Article › peer-review

Open Access

64 Scopus Citations

Sugar Release and Growth of Biofuel Crops are Improved by Downregulation of Pectin Biosynthesis

Biswal, A. K., Atmodjo, M. A., Li, M., Baxter, H. L., Yoo, C. G., Pu, Y., Lee, Y. C., Mazarei, M., Black, I. M., Zhang, J. Y., Ramanna, H., Bray, A. L., King, Z. R., Lafayette, P. R., Pattathil, S., Donohoe, B. S., Mohanty, S. S., Ryno, D., Yee, K. & Thompson, O. A. & 22 others, Rodriguez, M., Dumitrache, A., Natzke, J., Winkeler, K., Collins, C., Yang, X., Tan, L., Sykes, R. W., Gjersing, E. L., Ziebell, A., Turner, G. B., Decker, S. R., Hahn, M. G., Davison, B. H., Udvardi, M. K., Mielenz, J. R., Davis, M. F., Nelson, R. S., Parrott, W. A., Ragauskas, A. J., Neal Stewart, C. & Mohnen, D., 1 Mar 2018, In: Nature Biotechnology. 36, 3, p. 249-257 9 p.

Research output: Contribution to journal › Article › peer-review

125 Scopus Citations

Toward an Understanding of the Increase in Enzymatic Hydrolysis by Mechanical Refining

Donohoe, B., Assis, T., Huang, S., Driemeier, C., Kim, C., Kim, S., Gonzalez, R., Jameel, H. & Park, S., 2018, In: Biotechnology for Biofuels. 11, 1, 11 p., 289.

Research output: Contribution to journal › Article › peer-review

Open Access

34 Scopus Citations

2017

Adding Tetrahydrofuran to Dilute Acid Pretreatment Provides New Insights into Substrate Changes that Greatly Enhance Biomass Deconstruction by Clostridium thermocellum and Fungal Enzymes

Donohoe, B., Thomas, V., Li, M., Pu, Y., Ragauskas, A., Kumar, R., Nguyen, T., Cai, C. & Wyman, C., 2017, In: Biotechnology for Biofuels. 10, 1, 13 p., 252.

Research output: Contribution to journal › Article › peer-review

Open Access

41 Scopus Citations

Alkaline Peroxide Delignification of Corn Stover

Mittal, A., Katahira, R., Donohoe, B., Pleitner, B., Beckham, G., Pattathil, S. & Stringer, J., 3 Jul 2017, In: ACS Sustainable Chemistry and Engineering. 5, 7, p. 6310-6321 12 p.

Research output: Contribution to journal › Article › peer-review

68 Scopus Citations

Ammonia Pretreatment of Corn Stover Enables Facile Lignin Extraction

Mittal, A., Katahira, R., Donohoe, B., Nolker, M., Beckham, G., Pattathil, S., Kandemkavil, S. & Biddy, M., 6 Mar 2017, In: ACS Sustainable Chemistry and Engineering. 5, 3, p. 2544-2561 18 p.

Research output: Contribution to journal › Article › peer-review

79 Scopus Citations

Enhanced Rates of Enzymatic Saccharification and Catalytic Synthesis of Biofuel Substrates in Gelatinized Cellulose Generated by Trifluoroacetic Acid

Shiga, T. M., Xiao, W., Yang, H., Zhang, X., Olek, A. T., Donohoe, B. S., Liu, J., Makowski, L., Hou, T., McCann, M. C., Carpita, N. C. & Mosier, N. S., 2017, In: Biotechnology for Biofuels. 10, 1, 15 p., 310.

Research output: Contribution to journal › Article › peer-review

Open Access

23 Scopus Citations

Estimating the Temperature Experienced by Biomass Particles during Fast Pyrolysis Using Microscopic Analysis of Biochars

Donohoe, B., Ciesielski, P., Mukarakate, C., Nimlos, M., Thompson, L. & Jarvis, M., 17 Aug 2017, In: Energy and Fuels. 31, 8, p. 8193-8201 9 p.

Research output: Contribution to journal › Article › peer-review

8 Scopus Citations

Evaluation of Parameters Affecting Switchgrass Tissue Culture: Toward a Consolidated Procedure for Agrobacterium-Mediated Transformation of Switchgrass (Panicum virgatum)

Wei, H., Donohoe, B., Himmel, M., Ahuja, N., Garrity, D., Qu, R., Lin, C.-Y. & Tucker, M., 2017, In: Plant Methods. 13, 1, 19 p., 113.

Research output: Contribution to journal › Article › peer-review

Open Access

14 Scopus Citations

Lignocellulose Deconstruction in the Biosphere

Bomble, Y., Wei, H., Ciesielski, P., Donohoe, B., Decker, S., Himmel, M., Holwerda, E., Lynd, L., Lin, C.-Y. & Amore, A., Dec 2017, In: Current Opinion in Chemical Biology. 41, p. 61-70 10 p.

Research output: Contribution to journal › Article › peer-review

Open Access

98 Scopus Citations

The Multi Domain Caldicellulosiruptor bescii CelA Cellulase Excels at the Hydrolysis of Crystalline Cellulose

Bomble, Y., Brunecky, R., Donohoe, B., Yarbrough, J., Mittal, A., Chung, D., Himmel, M., Scott, B., Ding, H., Taylor III, L., Russell, J., Westpheling, J. & Teter, S., 1 Dec 2017, In: Scientific Reports. 7, 1, 17 p., Article No. 9622.

Research output: Contribution to journal › Article › peer-review

Open Access

42 Scopus Citations

Towards an Understanding of Enhanced Biomass Digestibility by In Planta Expression of a Family 5 Glycoside Hydrolase

Brunecky, R., Donohoe, B., Wei, H., Mittal, A., Himmel, M., Lunin, V. & Shollenberger, T., 1 Dec 2017, In: Scientific Reports. 7, 1, 9 p., Article No. 4389.

Research output: Contribution to journal › Article › peer-review

Open Access

10 Scopus Citations

2016

Biological Lignocellulose Solubilization: Comparative Evaluation of Biocatalysts and Enhancement via Cotreatment

Gjersing, E., Davis, M., Olstad-Thompson, J., Donohoe, B., Paye, J., Guseva, A., Hammer, S., Davison, B., Nguyen, T., Wyman, C., Pattathil, S., Hahn, M. & Lynd, L., 12 Jan 2016, In: Biotechnology for Biofuels. 9, 1, 13 p., 8.

Research output: Contribution to journal › Article › peer-review

Open Access

82 Scopus Citations

Directed Plant Cell-Wall Accumulation of Iron: Embedding Co-Catalyst for Efficient Biomass Conversion

Lin, C. Y., Jakes, J. E., Donohoe, B. S., Ciesielski, P. N., Yang, H., Gleber, S. C., Vogt, S., Ding, S. Y., Peer, W. A., Murphy, A. S., McCann, M. C., Himmel, M. E., Tucker, M. P. & Wei, H., 2016, In: Biotechnology for Biofuels. 9, 1, 15 p., 225.

Research output: Contribution to journal › Article › peer-review

Open Access

13 Scopus Citations

Dramatic Performance of Clostridium thermocellum Explained by its Wide Range of Cellulase Modalities

Xu, Q., Resch, M. G., Podkaminer, K., Yang, S., Baker, J. O., Donohoe, B. S., Wilson, C., Klingeman, D. M., Olson, D. G., Decker, S. R., Giannone, R. J., Hettich, R. L., Brown, S. D., Lynd, L. R., Bayer, E. A., Himmel, M. E. & Bomble, Y. J., 2016, In: Science Advances. 2, 2, 12 p., e1501254.

Research output: Contribution to journal › Article › peer-review

Open Access

95 Scopus Citations

Influence of Crystal Allomorph and Crystallinity on the Products and Behavior of Cellulose during Fast Pyrolysis

Mukarakate, C., Mittal, A., Ciesielski, P., Budhi, S., Thompson, L., Iisa, M., Nimlos, M. & Donohoe, B., 6 Sep 2016, In: ACS Sustainable Chemistry and Engineering. 4, 9, p. 4662-4674 13 p.

Research output: Contribution to journal › Article › peer-review

77 Scopus Citations

Interrelationships Between Cellulase Activity and Cellulose Particle Morphology.

Resch, M., Donohoe, B., Olsen, J., Borch, K. & Westh, P., 1 Aug 2016, In: Cellulose. 23, 4, p. 2349-2361 13 p.

Research output: Contribution to journal › Article › peer-review

9 Scopus Citations

Bryon Donohoe

Research Output

Search results