A 1.5 A Resolution X-Ray Structure of the Catalytic Module of Caldicellulosiruptor bescii Family 3 Pectate Lyase

Markus Alahuhta; Puja Chandrayan; Irina Kataeva; Michael W.W. Adams; Michael E. Himmel; Vladimir V. Lunin

doi:10.1107/S1744309111038449

A 1.5 A Resolution X-Ray Structure of the Catalytic Module of Caldicellulosiruptor bescii Family 3 Pectate Lyase

Markus Alahuhta, Puja Chandrayan, Irina Kataeva, Michael W.W. Adams, Michael E. Himmel, Vladimir V. Lunin

Research output: Contribution to journal › Article › peer-review

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Abstract

A 1.5 Å resolution X-ray structure of the catalytic module of Caldicellulosi-ruptor bescii family 3 pectate lyase is reported (PDB entry 3t9g). The resulting structure was refined to an R factor of 0.143 and an R free of 0.178. Structural analysis shows that this new structure is very similar to the previously solved structure of a family 3 pectate lyase from Bacillus sp. strain KSM-P15 (PDB entry 1ee6), with a root-mean-square deviation of 0.93 Å and a sequence identity of 53%. This structural similarity is significant considering that C. bescii is a hyperthermophile and Bacillus sp. is a mesophile.

Original language	American English
Pages (from-to)	1498-1500
Number of pages	3
Journal	Acta Crystallographica Section F: Structural Biology and Crystallization Communications
Volume	67
Issue number	12
DOIs	https://doi.org/10.1107/S1744309111038449 https://doi.org/10.1107/S1744309111038449
State	Published - Dec 2011

NREL Publication Number

NREL/JA-2700-52570

Keywords

Caldicellulosiruptor bescii
pectate lyases
PL3

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Alahuhta, M., Chandrayan, P., Kataeva, I., Adams, M. W. W., Himmel, M. E., & Lunin, V. V. (2011). A 1.5 A Resolution X-Ray Structure of the Catalytic Module of Caldicellulosiruptor bescii Family 3 Pectate Lyase. Acta Crystallographica Section F: Structural Biology and Crystallization Communications, 67(12), 1498-1500. https://doi.org/10.1107/S1744309111038449, https://doi.org/10.1107/S1744309111038449

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title = "A 1.5 A Resolution X-Ray Structure of the Catalytic Module of Caldicellulosiruptor bescii Family 3 Pectate Lyase",

abstract = "A 1.5 {\AA} resolution X-ray structure of the catalytic module of Caldicellulosi-ruptor bescii family 3 pectate lyase is reported (PDB entry 3t9g). The resulting structure was refined to an R factor of 0.143 and an R free of 0.178. Structural analysis shows that this new structure is very similar to the previously solved structure of a family 3 pectate lyase from Bacillus sp. strain KSM-P15 (PDB entry 1ee6), with a root-mean-square deviation of 0.93 {\AA} and a sequence identity of 53%. This structural similarity is significant considering that C. bescii is a hyperthermophile and Bacillus sp. is a mesophile.",

keywords = "Caldicellulosiruptor bescii, pectate lyases, PL3",

author = "Markus Alahuhta and Puja Chandrayan and Irina Kataeva and Adams, {Michael W.W.} and Himmel, {Michael E.} and Lunin, {Vladimir V.}",

year = "2011",

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doi = "10.1107/S1744309111038449",

language = "American English",

volume = "67",

pages = "1498--1500",

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Alahuhta, M, Chandrayan, P, Kataeva, I, Adams, MWW, Himmel, ME & Lunin, VV 2011, 'A 1.5 A Resolution X-Ray Structure of the Catalytic Module of Caldicellulosiruptor bescii Family 3 Pectate Lyase', Acta Crystallographica Section F: Structural Biology and Crystallization Communications, vol. 67, no. 12, pp. 1498-1500. https://doi.org/10.1107/S1744309111038449, https://doi.org/10.1107/S1744309111038449

A 1.5 A Resolution X-Ray Structure of the Catalytic Module of Caldicellulosiruptor bescii Family 3 Pectate Lyase. / Alahuhta, Markus; Chandrayan, Puja; Kataeva, Irina et al.
In: Acta Crystallographica Section F: Structural Biology and Crystallization Communications, Vol. 67, No. 12, 12.2011, p. 1498-1500.

Research output: Contribution to journal › Article › peer-review

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T1 - A 1.5 A Resolution X-Ray Structure of the Catalytic Module of Caldicellulosiruptor bescii Family 3 Pectate Lyase

AU - Alahuhta, Markus

AU - Chandrayan, Puja

AU - Kataeva, Irina

AU - Adams, Michael W.W.

AU - Himmel, Michael E.

AU - Lunin, Vladimir V.

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AB - A 1.5 Å resolution X-ray structure of the catalytic module of Caldicellulosi-ruptor bescii family 3 pectate lyase is reported (PDB entry 3t9g). The resulting structure was refined to an R factor of 0.143 and an R free of 0.178. Structural analysis shows that this new structure is very similar to the previously solved structure of a family 3 pectate lyase from Bacillus sp. strain KSM-P15 (PDB entry 1ee6), with a root-mean-square deviation of 0.93 Å and a sequence identity of 53%. This structural similarity is significant considering that C. bescii is a hyperthermophile and Bacillus sp. is a mesophile.

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A 1.5 A Resolution X-Ray Structure of the Catalytic Module of Caldicellulosiruptor bescii Family 3 Pectate Lyase

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