A Multi-Omic Characterization of Temperature Stress in a Halotolerant Scenedesmus Strain for Algal Biotechnology

Sara Calhoun; Tisza Szeremy Bell; Lukas Dahlin; Yuliya Kunde; Kurt LaButti; Katherine Louie; Andrea Kuftin; Daniel Treen; David Dilworth; Sirma Mihaltcheva; Christopher Daum; Benjamin Bowen; Trent Northen; Michael Guarnieri; Shawn Starkenburg; Igor Grigoriev

doi:10.1038/s42003-021-01859-y

A Multi-Omic Characterization of Temperature Stress in a Halotolerant Scenedesmus Strain for Algal Biotechnology

Sara Calhoun, Tisza Szeremy Bell, Lukas Dahlin, Yuliya Kunde, Kurt LaButti, Katherine Louie, Andrea Kuftin, Daniel Treen, David Dilworth, Sirma Mihaltcheva, Christopher Daum, Benjamin Bowen, Trent Northen, Michael Guarnieri, Shawn Starkenburg, Igor Grigoriev

Biosciences Center

Research output: Contribution to journal › Article › peer-review

32 Scopus Citations

Abstract

Microalgae efficiently convert sunlight into lipids and carbohydrates, offering bio-based alternatives for energy and chemical production. Improving algal productivity and robustness against abiotic stress requires a systems level characterization enabled by functional genomics. Here, we characterize a halotolerant microalga Scenedesmus sp. NREL 46B-D3 demonstrating peak growth near 25 °C that reaches 30 g/m²/day and the highest biomass accumulation capacity post cell division reported to date for a halotolerant strain. Functional genomics analysis revealed that genes involved in lipid production, ion channels and antiporters are expanded and expressed. Exposure to temperature stress shifts fatty acid metabolism and increases amino acids synthesis. Co-expression analysis shows that many fatty acid biosynthesis genes are overexpressed with specific transcription factors under cold stress. These and other genes involved in the metabolic and regulatory response to temperature stress can be further explored for strain improvement.

Original language	American English
Article number	333
Number of pages	15
Journal	Communications Biology
Volume	4
Issue number	1
DOIs	https://doi.org/10.1038/s42003-021-01859-y https://doi.org/10.1038/s42003-021-01859-y
State	Published - Dec 2021

Bibliographical note

Publisher Copyright:
© 2021, This is a U.S. government work and not under copyright protection in the U.S.; foreign copyright protection may apply.

NREL Publication Number

NREL/JA-2700-78199

Keywords

biofuel algae
Chlorophyceae
genome
Scenedesmaceae

Access to Document

https://www.nrel.gov/docs/fy21osti/78199.pdf

Cite this

Calhoun, S., Szeremy Bell, T., Dahlin, L., Kunde, Y., LaButti, K., Louie, K., Kuftin, A., Treen, D., Dilworth, D., Mihaltcheva, S., Daum, C., Bowen, B., Northen, T., Guarnieri, M., Starkenburg, S., & Grigoriev, I. (2021). A Multi-Omic Characterization of Temperature Stress in a Halotolerant Scenedesmus Strain for Algal Biotechnology. Communications Biology, 4(1), Article 333. https://doi.org/10.1038/s42003-021-01859-y, https://doi.org/10.1038/s42003-021-01859-y

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abstract = "Microalgae efficiently convert sunlight into lipids and carbohydrates, offering bio-based alternatives for energy and chemical production. Improving algal productivity and robustness against abiotic stress requires a systems level characterization enabled by functional genomics. Here, we characterize a halotolerant microalga Scenedesmus sp. NREL 46B-D3 demonstrating peak growth near 25 °C that reaches 30 g/m2/day and the highest biomass accumulation capacity post cell division reported to date for a halotolerant strain. Functional genomics analysis revealed that genes involved in lipid production, ion channels and antiporters are expanded and expressed. Exposure to temperature stress shifts fatty acid metabolism and increases amino acids synthesis. Co-expression analysis shows that many fatty acid biosynthesis genes are overexpressed with specific transcription factors under cold stress. These and other genes involved in the metabolic and regulatory response to temperature stress can be further explored for strain improvement.",

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Calhoun, S, Szeremy Bell, T, Dahlin, L, Kunde, Y, LaButti, K, Louie, K, Kuftin, A, Treen, D, Dilworth, D, Mihaltcheva, S, Daum, C, Bowen, B, Northen, T, Guarnieri, M, Starkenburg, S & Grigoriev, I 2021, 'A Multi-Omic Characterization of Temperature Stress in a Halotolerant Scenedesmus Strain for Algal Biotechnology', Communications Biology, vol. 4, no. 1, 333. https://doi.org/10.1038/s42003-021-01859-y, https://doi.org/10.1038/s42003-021-01859-y

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T1 - A Multi-Omic Characterization of Temperature Stress in a Halotolerant Scenedesmus Strain for Algal Biotechnology

AU - Calhoun, Sara

AU - Szeremy Bell, Tisza

AU - Dahlin, Lukas

AU - Kunde, Yuliya

AU - LaButti, Kurt

AU - Louie, Katherine

AU - Kuftin, Andrea

AU - Treen, Daniel

AU - Dilworth, David

AU - Mihaltcheva, Sirma

AU - Daum, Christopher

AU - Bowen, Benjamin

AU - Northen, Trent

AU - Guarnieri, Michael

AU - Starkenburg, Shawn

AU - Grigoriev, Igor

PY - 2021/12

Y1 - 2021/12

N2 - Microalgae efficiently convert sunlight into lipids and carbohydrates, offering bio-based alternatives for energy and chemical production. Improving algal productivity and robustness against abiotic stress requires a systems level characterization enabled by functional genomics. Here, we characterize a halotolerant microalga Scenedesmus sp. NREL 46B-D3 demonstrating peak growth near 25 °C that reaches 30 g/m2/day and the highest biomass accumulation capacity post cell division reported to date for a halotolerant strain. Functional genomics analysis revealed that genes involved in lipid production, ion channels and antiporters are expanded and expressed. Exposure to temperature stress shifts fatty acid metabolism and increases amino acids synthesis. Co-expression analysis shows that many fatty acid biosynthesis genes are overexpressed with specific transcription factors under cold stress. These and other genes involved in the metabolic and regulatory response to temperature stress can be further explored for strain improvement.

AB - Microalgae efficiently convert sunlight into lipids and carbohydrates, offering bio-based alternatives for energy and chemical production. Improving algal productivity and robustness against abiotic stress requires a systems level characterization enabled by functional genomics. Here, we characterize a halotolerant microalga Scenedesmus sp. NREL 46B-D3 demonstrating peak growth near 25 °C that reaches 30 g/m2/day and the highest biomass accumulation capacity post cell division reported to date for a halotolerant strain. Functional genomics analysis revealed that genes involved in lipid production, ion channels and antiporters are expanded and expressed. Exposure to temperature stress shifts fatty acid metabolism and increases amino acids synthesis. Co-expression analysis shows that many fatty acid biosynthesis genes are overexpressed with specific transcription factors under cold stress. These and other genes involved in the metabolic and regulatory response to temperature stress can be further explored for strain improvement.

KW - biofuel algae

KW - Chlorophyceae

KW - genome

KW - Scenedesmaceae

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ER -

A Multi-Omic Characterization of Temperature Stress in a Halotolerant Scenedesmus Strain for Algal Biotechnology

Abstract

Bibliographical note

NREL Publication Number

Keywords

Access to Document

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