A Novel Integrated Biorefinery Process for Diesel Fuel Blendstock Production using Lipids from the Methanotroph, Methylomicrobium buryatense

Tao Dong, Qiang Fei, Marie Genelot, Holly Smith, Lieve M.L. Laurens, Michael J. Watson, Philip T. Pienkos

Research output: Contribution to journalArticlepeer-review

53 Scopus Citations

Abstract

In light of the availability of low-cost methane (CH4) derived from natural gas and biogas along with increasing concerns of the greenhouse gas emissions, the production of alternative liquid biofuels directly from CH4 is a promising approach to capturing wasted energy. A novel biorefinery concept integrating biological conversion of CH4 to microbial lipids together with lipid extraction and generation of hydrocarbon fuels is demonstrated in this study for the first time. An aerobic methanotrophic bacterium, Methylomicrobium buryatense capable of using CH4 as the sole carbon source was selected on the basis of genetic tractability, cultivation robustness, and ability to accumulate phospholipids in membranes. A maximum fatty acid content of 10% of dry cell weight was obtained in batch cultures grown in a continuous gas sparging fermentation system. Although phospholipids are not typically considered as a good feedstock for upgrading to hydrocarbon fuels, we set out to demonstrate that using a combination of novel lipid extraction methodology with advanced catalyst design, we could prove the feasibility of this approach. Up to 95% of the total fatty acids from membrane-bound phospholipids were recovered by a two-stage pretreatment method followed by hexane extraction of the aqueous hydrolysate. The upgrading of extracted lipids was then demonstrated in a hydrodeoxygeation process using palladium on silica as a catalyst. Lipid conversion in excess of 99% was achieved, with a full selectivity to hydrocarbons. The final hydrocarbon mixture is dominated by 88% pentadecane (C15H32) based on decarbonylation/decarboxylation and hydrogenation of C16 fatty acids, indicating that a biological gas-to-liquid fuel (Bio-GTL) process is technically feasible.

Original languageAmerican English
Pages (from-to)62-70
Number of pages9
JournalEnergy Conversion and Management
Volume140
DOIs
StatePublished - 2017

Bibliographical note

Publisher Copyright:
© 2017 The Authors

NREL Publication Number

  • NREL/JA-5100-65377

Keywords

  • Biofuels
  • Biorefinery
  • Extraction
  • Fermentation
  • Hydrotreating
  • Methahnotroph

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