A Previously Uncharacterized Divisome-Associated Lipoprotein, DalA, is Needed for Normal Cell Division in Rhodobacterales: Article No. e01203-23

Francois Alberge, Bryan Lakey, Ryan Schaub, Alice Dohnalkova, Kimberley Lemmer, Joseph Dillard, Daniel Noguera, Timothy Donohue

Research output: Contribution to journalArticlepeer-review

Abstract

The bacterial cell envelope is a key subcellular compartment with important roles in antibiotic resistance, nutrient acquisition, and cell morphology. We seek to gain a better understanding of proteins that contribute to the function of the cell envelope in Alphaproteobacteria. Using Rhodobacter sphaeroides, we show that a previously uncharacterized protein, RSP_1200, is an outer membrane (OM) lipoprotein that non-covalently binds peptidoglycan (PG). Using a fluorescently tagged version of this protein, we find that RSP_1200 undergoes a dynamic repositioning during the cell cycle and is enriched at the septum during cell division. We show that the position of RSP_1200 mirrors the location of FtsZ rings, leading us to propose that RSP_1200 is a newly identified component of the R. sphaeroides' divisome. Additional support for this hypothesis includes the co-precipitation of RSP_1200 with FtsZ, the Pal protein, and several predicted PG L,D-transpeptidases. We also find that a RSP_1200 mutation leads to defects in cell division, sensitivity to PG-active antibiotics, and results in the formation of OM protrusions at the septum during cell division. Based on these results, we propose to name RSP_1200 DalA (for division-associated lipoprotein A) and postulate that DalA serves as a scaffold to position or modulate the activity of PG transpeptidases that are needed to form envelope invaginations during cell division. We find that DalA homologs are present in members of the Rhodobacterales order within Alphaproteobacteria. Therefore, we propose that further analysis of this and related proteins will increase our understanding of the macromolecular machinery and proteins that participate in cell division in Gram-negative bacteria.
Original languageAmerican English
Number of pages24
JournalmBio
Volume14
Issue number4
DOIs
StatePublished - 2023

NREL Publication Number

  • NREL/JA-6A20-86957

Keywords

  • Alphaproteobacteria
  • cell division
  • cell envelope
  • lipoprotein
  • peptidoglycan

Fingerprint

Dive into the research topics of 'A Previously Uncharacterized Divisome-Associated Lipoprotein, DalA, is Needed for Normal Cell Division in Rhodobacterales: Article No. e01203-23'. Together they form a unique fingerprint.

Cite this