A Thioacidolysis Method Tailored for Higher-Throughput Quantitative Analysis of Lignin Monomers

Anne Ware, Renee Happs, Crissa Doeppke, Mark Davis, Clifford Foster, Kristoffer Meunier, Jackson Gehan, Fengxia Yue, Fachuang Lu

Research output: Contribution to journalArticlepeer-review

35 Scopus Citations

Abstract

Thioacidolysis is a method used to measure the relative content of lignin monomers bound by β-O-4 linkages. Current thioacidolysis methods are low-throughput as they require tedious steps for reaction product concentration prior to analysis using standard GC methods. A quantitative thioacidolysis method that is accessible with general laboratory equipment and uses a non-chlorinated organic solvent and is tailored for higher-throughput analysis is reported. The method utilizes lignin arylglycerol monomer standards for calibration, requires 1–2 mg of biomass per assay and has been quantified using fast-GC techniques including a Low Thermal Mass Modular Accelerated Column Heater (LTM MACH). Cumbersome steps, including standard purification, sample concentrating and drying have been eliminated to help aid in consecutive day-to-day analyses needed to sustain a high sample throughput for large screening experiments without the loss of quantitation accuracy. The method reported in this manuscript has been quantitatively validated against a commonly used thioacidolysis method and across two different research sites with three common biomass varieties to represent hardwoods, softwoods, and grasses.

Original languageAmerican English
Pages (from-to)1268-1273
Number of pages6
JournalBiotechnology Journal
Volume11
Issue number10
DOIs
StatePublished - 2016

Bibliographical note

Publisher Copyright:
© 2016 The Authors. Biotechnology Journal published by WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

NREL Publication Number

  • NREL/JA-5100-65297

Keywords

  • Cell wall structure
  • Lignin
  • S/G ratio
  • Thioacidolysis

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