Accounting for All Sugars Produced during Integrated Production of Ethanol from Lignocellulosic Biomass

Research output: Contribution to journalArticlepeer-review

19 Scopus Citations


Accurate mass balance and conversion data from integrated operation is needed to fully elucidate the economics of biofuel production processes. This study explored integrated conversion of corn stover to ethanol and highlights techniques for accurate yield calculations. Acid pretreated corn stover (PCS) produced in a pilot-scale reactor was enzymatically hydrolyzed and the resulting sugars were fermented to ethanol by the glucose-xylose fermenting bacteria, Zymomonas mobilis 8b. The calculations presented here account for high solids operation and oligomeric sugars produced during pretreatment, enzymatic hydrolysis, and fermentation, which, if not accounted for, leads to overestimating ethanol yields. The calculations are illustrated for enzymatic hydrolysis and fermentation of PCS at 17.5% and 20.0% total solids achieving 80.1% and 77.9% conversion of cellulose and xylan to ethanol and ethanol titers of 63 g/L and 69 g/L, respectively. These procedures will be employed in the future and the resulting information used for techno-economic analysis.

Original languageAmerican English
Pages (from-to)153-158
Number of pages6
JournalBioresource Technology
StatePublished - 2016

Bibliographical note

Publisher Copyright:
© 2016 Elsevier Ltd.

NREL Publication Number

  • NREL/JA-5100-65494


  • Enzymatic hydrolysis
  • Ethanol
  • Fermentation
  • Pretreatment
  • Yields


Dive into the research topics of 'Accounting for All Sugars Produced during Integrated Production of Ethanol from Lignocellulosic Biomass'. Together they form a unique fingerprint.

Cite this