Adsorption of Cellobiohydrolases I onto Lignin Fractions from Dilute Acid Pretreated Broussonetia papyrifera

Robert Sykes, Lan Yao, Haitao Yang, Chang Yoo, Xianzhi Meng, Mi Li, Yunqiao Pu, Arthur Ragauskas

Research output: Contribution to journalArticlepeer-review

28 Scopus Citations

Abstract

Broussonetia papyrifera, known as paper mulberry, is a potential feed stock for bioethanol production because of its cellulose-rich composition. Lignin in dilute acid pretreated Broussonetia papyrifera was fractionated to three different fractions, and their physiochemical properties were determined by FT-IR, GPC and NMR analyses. Different structural characteristics were observed from each lignin fraction. Cellobiohydrolases I (CBH) adsorption to each lignin was understood by the lignin properties. The results showed that aliphatic hydroxyl groups in lignin showed positive correlations with the maximum binding ability of CBH onto lignin samples. Also, the contents of phenolic compounds such as p-hydroxyphenyl benzoate (PB), syringyl (S) and guaiacyl (G) units in the lignin influenced their CBH binding.
Original languageAmerican English
Pages (from-to)957-962
Number of pages6
JournalBioresource Technology
Volume244
Issue numberPart 1
DOIs
StatePublished - 2017

NREL Publication Number

  • NREL/JA-5100-70099

Keywords

  • adsorption
  • Broussonetia papyrifera
  • cellobiohydrolases I
  • dilute acid
  • lignin fraction
  • pretreatment

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