Advances in Integrative Structural Biology: Towards Understanding Protein Complexes in their Cellular Context

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21 Scopus Citations

Abstract

Microorganisms rely on protein interactions to transmit signals, react to stimuli, and grow. One of the best ways to understand these protein interactions is through structural characterization. However, in the past, structural knowledge was limited to stable, high-affinity complexes that could be crystallized. Recent developments in structural biology have revolutionized how protein interactions are characterized. The combination of multiple techniques, known as integrative structural biology, has provided insight into how large protein complexes interact in their native environment. In this mini-review, we describe the past, present, and potential future of integrative structural biology as a tool for characterizing protein interactions in their cellular context.

Original languageAmerican English
Pages (from-to)214-225
Number of pages12
JournalComputational and Structural Biotechnology Journal
Volume19
DOIs
StatePublished - Jan 2021

Bibliographical note

Publisher Copyright:
© 2020 The Authors

NREL Publication Number

  • NREL/JA-2700-78578

Keywords

  • Crosslinking mass spectrometry
  • Cryo-electron microscopy
  • Cryo-electron tomography
  • Integrative structural biology
  • Protein docking
  • Protein structure prediction
  • Quinary interactions
  • X-ray crystallography

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