Analysis of Oligomeric Fermentation Substrates and Products by High Pressure Size Exclusion Chromatography

K. Oh; J. Janssens; K. Grohmann; M. E. Himmel

doi:10.1007/BF01134586

Analysis of Oligomeric Fermentation Substrates and Products by High Pressure Size Exclusion Chromatography

K. Oh, J. Janssens, K. Grohmann, M. E. Himmel

Bioenergy Science and Technology

Research output: Contribution to journal › Article › peer-review

Abstract

Commercially available maltodextrins were subjected to high pressure size exclusion chromatography (HPSEC) on Toyo Soda G 2000 PW columns with water as the mobile phase. The elution profiles of these samples will allow researchers to select the correct maltodextrin for growth studies requiring specific dextrin oligomers. Characterization of the chromatography system with standards of known molecular weight allows estimation of the weight average molecular weight of polydisperse dextrins. The enzymatic hydrolysis of dextrin by bacterial α-amylase and fungal glucoamylase was also monitored by HPSEC.

Original language	American English
Pages (from-to)	405-410
Number of pages	6
Journal	Biotechnology Letters
Volume	4
Issue number	7
DOIs	https://doi.org/10.1007/BF01134586 https://doi.org/10.1007/BF01134586
State	Published - 1982

NREL Publication Number

ACNR/JA-232-5015

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abstract = "Commercially available maltodextrins were subjected to high pressure size exclusion chromatography (HPSEC) on Toyo Soda G 2000 PW columns with water as the mobile phase. The elution profiles of these samples will allow researchers to select the correct maltodextrin for growth studies requiring specific dextrin oligomers. Characterization of the chromatography system with standards of known molecular weight allows estimation of the weight average molecular weight of polydisperse dextrins. The enzymatic hydrolysis of dextrin by bacterial α-amylase and fungal glucoamylase was also monitored by HPSEC.",

author = "K. Oh and J. Janssens and K. Grohmann and Himmel, {M. E.}",

year = "1982",

doi = "10.1007/BF01134586",

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pages = "405--410",

journal = "Biotechnology Letters",

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AU - Oh, K.

AU - Janssens, J.

AU - Grohmann, K.

AU - Himmel, M. E.

PY - 1982

Y1 - 1982

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AB - Commercially available maltodextrins were subjected to high pressure size exclusion chromatography (HPSEC) on Toyo Soda G 2000 PW columns with water as the mobile phase. The elution profiles of these samples will allow researchers to select the correct maltodextrin for growth studies requiring specific dextrin oligomers. Characterization of the chromatography system with standards of known molecular weight allows estimation of the weight average molecular weight of polydisperse dextrins. The enzymatic hydrolysis of dextrin by bacterial α-amylase and fungal glucoamylase was also monitored by HPSEC.

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AN - SCOPUS:34250228106

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EP - 410

JO - Biotechnology Letters

JF - Biotechnology Letters

IS - 7

ER -

Analysis of Oligomeric Fermentation Substrates and Products by High Pressure Size Exclusion Chromatography

Abstract

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