Abstract

Carboxylic acid reductases (CARs) are recently emerging reductive enzymes for the direct production of aldehydes from biologically-produced carboxylic acids. Recent work has demonstrated that these powerful enzymes are able to reduce a very broad range of volatile- to long-chain fatty acids as well as aromatic acids. Here, we express four CAR enzymes from different fungal origins to test their activity against fatty acids commonly produced in oleaginous microbes. These in vitro results will inform metabolic engineering strategies to conduct mild biological reduction of carboxylic acids in situ, which is conventionally done via hydrotreating catalysis at high temperatures and hydrogen pressures.
Original languageAmerican English
StatePublished - 2016

Publication series

NamePresented at the Symposium on Biotechnology for Fuels and Chemicals (SBFC), 25-28 April 2016, Baltimore, Maryland

NREL Publication Number

  • NREL/PO-5100-66304

Keywords

  • biomass
  • carboxylic acid reductase
  • fatty acids
  • fuels

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