Approaches to Developing Biological H2-Photoproducing Organisms and Processes

M. L. Ghirardi; P. W. King; M. C. Posewitz; P. Ching Maness; A. Fedorov; K. Kim; J. Cohen; K. Schulten; M. Seibert

doi:10.1042/BST0330070

Approaches to Developing Biological H2-Photoproducing Organisms and Processes

M. L. Ghirardi, P. W. King, M. C. Posewitz, P. Ching Maness, A. Fedorov, K. Kim, J. Cohen, K. Schulten, M. Seibert

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Abstract

The development of efficient biological systems for the direct photoproduction of H₂ gas from water faces several challenges, the more serious of which is the sensitivity of the H₂-evolving enzymes (hydrogenases) to O₂, an obligatory by-product of photosynthesis. This high sensitivity is common to both FeFe and NiFe hydrogenases, and is caused by O₂ binding to their respective metallocatalytic sites. This overview describes approaches to (i) molecular engineering of algal FeFe-hydrogenase to prevent O₂ access to its catalytic site; (ii) transform a cyanobacterium with an O₂-tolerant bacterial NiFe hydrogenase or (c) partially inactivate algal O₂-evolution activity to create physiologically anaerobiosis and induce hydrogenase expression.

Original language	American English
Pages (from-to)	70-72
Number of pages	3
Journal	Biochemical Society Transactions
Volume	33
Issue number	1
DOIs	https://doi.org/10.1042/BST0330070 https://doi.org/10.1042/BST0330070
State	Published - 2005

NREL Publication Number

NREL/JA-590-38437

Keywords

Biohydrogen
Cyanobacteria
Green algae
Hydrogenase
O inactivation
Sulphur deprivation

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title = "Approaches to Developing Biological H2-Photoproducing Organisms and Processes",

abstract = "The development of efficient biological systems for the direct photoproduction of H2 gas from water faces several challenges, the more serious of which is the sensitivity of the H2-evolving enzymes (hydrogenases) to O2, an obligatory by-product of photosynthesis. This high sensitivity is common to both FeFe and NiFe hydrogenases, and is caused by O2 binding to their respective metallocatalytic sites. This overview describes approaches to (i) molecular engineering of algal FeFe-hydrogenase to prevent O2 access to its catalytic site; (ii) transform a cyanobacterium with an O2-tolerant bacterial NiFe hydrogenase or (c) partially inactivate algal O2-evolution activity to create physiologically anaerobiosis and induce hydrogenase expression.",

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AU - Ghirardi, M. L.

AU - King, P. W.

AU - Posewitz, M. C.

AU - Maness, P. Ching

AU - Fedorov, A.

AU - Kim, K.

AU - Cohen, J.

AU - Schulten, K.

AU - Seibert, M.

PY - 2005

Y1 - 2005

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AB - The development of efficient biological systems for the direct photoproduction of H2 gas from water faces several challenges, the more serious of which is the sensitivity of the H2-evolving enzymes (hydrogenases) to O2, an obligatory by-product of photosynthesis. This high sensitivity is common to both FeFe and NiFe hydrogenases, and is caused by O2 binding to their respective metallocatalytic sites. This overview describes approaches to (i) molecular engineering of algal FeFe-hydrogenase to prevent O2 access to its catalytic site; (ii) transform a cyanobacterium with an O2-tolerant bacterial NiFe hydrogenase or (c) partially inactivate algal O2-evolution activity to create physiologically anaerobiosis and induce hydrogenase expression.

KW - Biohydrogen

KW - Cyanobacteria

KW - Green algae

KW - Hydrogenase

KW - O inactivation

KW - Sulphur deprivation

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Approaches to Developing Biological H2-Photoproducing Organisms and Processes

Abstract

NREL Publication Number

Keywords

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