Atrazine Degradation Using Immobilized Triazine Hydrolase from Arthrobacter aurescens TC1 in Mesoporous Silica Nanomaterials

Karla Diviesti, Glory Russell-Parks, Brian Trewyn, Richard Holz

Research output: Contribution to journalArticlepeer-review

Abstract

Triazine hydrolase fromArthrobacter aurescens TC1 (TrzN) was successfully immobilized on mesoporous silica nanomaterials (MSNs) for the first time. For both nonfunctionalized MSNs and MSNs functionalized with Zn(II), three pore sizes were evaluated for their ability to immobilize wild-type TrzN: Mobile composition of matter no. 41 (small, 3 nm pores), mesoporous silica nanoparticle material with 10 nm pore diameter (MSN-10) (medium, 6-12 nm pores), and pore-expanded MSN-10 (large, 15-30 nm pores). Of these six TrzN:MSN biomaterials, it was shown that TrzN:MSN-10 was the most active (3.8 +/- 0.4 x 10-5 U/mg) toward the hydrolysis of a 50 uM atrazine solution at 25 degrees C. The TrzN:MSN-10 biomaterial was then coated in chitosan (TrzN:MSN-10:Chit) as chitosan has been shown to increase stability in extreme conditions such as low/high pH, heat shock, and the presence of organic solvents. TrzN:MSN-10:Chit was shown to be a superior TrzN biomaterial to TrzN:MSN-10 as it exhibited higher activity under all storage conditions, in the presence of 20% MeOH, at low and high pH values, and at elevated temperatures up to 80 degrees C. Finally, the TrzN:MSN-10:Chit biomaterial was shown to be fully active in river water, which establishes it as a functional biomaterial under actual field conditions. A combination of these data indicate that the TrzN:MSN-10:Chit biomaterial exhibited the best overall catalytic profile making it a promising biocatalyst for the bioremediation of atrazine.
Original languageAmerican English
Pages (from-to)361-369
Number of pages9
JournalACS Environmental Au
Volume3
Issue number6
DOIs
StatePublished - 2023

NREL Publication Number

  • NREL/JA-5900-88497

Keywords

  • biocatalysis
  • biomaterials
  • bioremediation
  • dehalogenase
  • kinetics
  • mesoporous silica nanoparticles
  • zinc

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