Azido [14C] Atrazine Photoaffinity Technique Labels a 34-kDa Protein in Scenedesmus which Functions on the Oxidizing Side of Photosystem II

James G. Metz; Terry M. Bricker; Michael Seibert

doi:10.1016/0014-5793(85)80768-7

Azido [14C] Atrazine Photoaffinity Technique Labels a 34-kDa Protein in Scenedesmus which Functions on the Oxidizing Side of Photosystem II

James G. Metz, Terry M. Bricker, Michael Seibert

Research output: Contribution to journal › Article › peer-review

34 Scopus Citations

Abstract

We have used azido[¹⁴C]atrazine to photoaffinity label thylakoids from wild-type (WT) Scenedesmus and a mutant, LF-1, which is blocked on the oxidizing side of photosystem II (PS II). One protein is labeled in each case, at 34 kDa in the WT and 36 kDa in LF-1. Previous comparison of the WT with LF-1 had been used to assign a PS II donor side function to the 34-kDa protein. These results suggest that this photoaffinity technique does not label the herbicide-binding protein involved in electron transfer on the reducing side of PS II.

Original language	American English
Pages (from-to)	191-196
Number of pages	6
Journal	FEBS Letters
Volume	185
Issue number	1
DOIs	https://doi.org/10.1016/0014-5793(85)80768-7 https://doi.org/10.1016/0014-5793(85)80768-7
State	Published - 1985
Externally published	Yes

NREL Publication Number

ACNR/JA-233-6344

Keywords

Azidoatrazine
Herbicide binding
LF-1 mutant
Oxygen evolution
Photosystem II
Scenedesmus

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title = "Azido [14C] Atrazine Photoaffinity Technique Labels a 34-kDa Protein in Scenedesmus which Functions on the Oxidizing Side of Photosystem II",

abstract = "We have used azido[14C]atrazine to photoaffinity label thylakoids from wild-type (WT) Scenedesmus and a mutant, LF-1, which is blocked on the oxidizing side of photosystem II (PS II). One protein is labeled in each case, at 34 kDa in the WT and 36 kDa in LF-1. Previous comparison of the WT with LF-1 had been used to assign a PS II donor side function to the 34-kDa protein. These results suggest that this photoaffinity technique does not label the herbicide-binding protein involved in electron transfer on the reducing side of PS II.",

keywords = "Azidoatrazine, Herbicide binding, LF-1 mutant, Oxygen evolution, Photosystem II, Scenedesmus",

author = "Metz, {James G.} and Bricker, {Terry M.} and Michael Seibert",

year = "1985",

doi = "10.1016/0014-5793(85)80768-7",

language = "American English",

volume = "185",

pages = "191--196",

journal = "FEBS Letters",

issn = "0014-5793",

publisher = "Wiley-Blackwell",

number = "1",

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T1 - Azido [14C] Atrazine Photoaffinity Technique Labels a 34-kDa Protein in Scenedesmus which Functions on the Oxidizing Side of Photosystem II

AU - Metz, James G.

AU - Bricker, Terry M.

AU - Seibert, Michael

PY - 1985

Y1 - 1985

N2 - We have used azido[14C]atrazine to photoaffinity label thylakoids from wild-type (WT) Scenedesmus and a mutant, LF-1, which is blocked on the oxidizing side of photosystem II (PS II). One protein is labeled in each case, at 34 kDa in the WT and 36 kDa in LF-1. Previous comparison of the WT with LF-1 had been used to assign a PS II donor side function to the 34-kDa protein. These results suggest that this photoaffinity technique does not label the herbicide-binding protein involved in electron transfer on the reducing side of PS II.

AB - We have used azido[14C]atrazine to photoaffinity label thylakoids from wild-type (WT) Scenedesmus and a mutant, LF-1, which is blocked on the oxidizing side of photosystem II (PS II). One protein is labeled in each case, at 34 kDa in the WT and 36 kDa in LF-1. Previous comparison of the WT with LF-1 had been used to assign a PS II donor side function to the 34-kDa protein. These results suggest that this photoaffinity technique does not label the herbicide-binding protein involved in electron transfer on the reducing side of PS II.

KW - Azidoatrazine

KW - Herbicide binding

KW - LF-1 mutant

KW - Oxygen evolution

KW - Photosystem II

KW - Scenedesmus

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DO - 10.1016/0014-5793(85)80768-7

M3 - Article

AN - SCOPUS:0040492020

SN - 0014-5793

VL - 185

SP - 191

EP - 196

JO - FEBS Letters

JF - FEBS Letters

IS - 1

ER -

Azido [14C] Atrazine Photoaffinity Technique Labels a 34-kDa Protein in Scenedesmus which Functions on the Oxidizing Side of Photosystem II

Abstract

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