Biological Conversion of Cyclic Ketones from Catalytic Fast Pyrolysis with Pseudomonas putida KT2440

Andrew Borchert, A. Wilson, William Michener, Joseph Roback, William Henson, Kelsey Ramirez, Gregg Beckham

Research output: Contribution to journalArticlepeer-review

2 Scopus Citations


Catalytic fast pyrolysis (CFP) of lignocellulosic biomass is under intense investigation to produce sustainable biofuels. CFP produces a heterogenous bio-oil fraction rich in oxygenated species, including cyclic ketones. While the high oxygen content of CFP bio-oil renders it unsuitable for direct use as transportation fuel replacement or blendstock, many oxygenates can be separated and upgraded to value-added chemicals to offset biofuels’ production cost. In this work, we isolated fractions enriched in cyclic ketones from ex situ CFP bio-oil and metabolically engineered the robust soil bacterium Pseudomonas putida KT2440 to upgrade these ketones to hydroxy and dicarboxylic acids, which have a broad set of industrial applications, especially for use in polymers. P. putida was adapted to higher concentrations of the cyclic ketone substrate, 2-cyclopenten-1-one, to further improve the conversion process. Overall, this work demonstrates the valorization of an important class of compounds found in CFP bio-oil, further expanding the possibilities of producing valuable bioproducts in CFP processes, especially from substrates that are disadvantaged for biofuels production.

Original languageAmerican English
Pages (from-to)3278-3291
Number of pages14
JournalGreen Chemistry
Issue number8
StatePublished - 2023

Bibliographical note

Publisher Copyright:
© 2023 The Royal Society of Chemistry.

NREL Publication Number

  • NREL/JA-2A00-85009


  • 5-hydroxyvaleric acid
  • catalytic fast pyrolysis
  • cyclopentanone
  • glutaric acid
  • Pseudomonas putida


Dive into the research topics of 'Biological Conversion of Cyclic Ketones from Catalytic Fast Pyrolysis with Pseudomonas putida KT2440'. Together they form a unique fingerprint.

Cite this