A Carboxylic Residue at the High-Affinity, Mn-Binding Site Participates in the Binding of Iron Cations that Block the Site

Boris K. Semin, Michael Seibert

Research output: Contribution to journalArticlepeer-review

12 Scopus Citations

Abstract

The role of carboxylic residues at the high-affinity, Mn-binding site in the ligation of iron cations blocking the site [Biochemistry 41 (2000) 5854] was studied, using a method developed to extract the iron cations blocking the site. We found that specifically bound Fe(III) cations can be extracted with citrate buffer at pH 3.0. Furthermore, citrate can also prevent the photooxidation of Fe(II) cations by YZ{radical dot}. Participation of a COOH group(s) in the ligation of Fe(III) at the high-affinity site was investigated using 1-ethyl-3-[(3-dimethylamino)propyl] carbodiimide (EDC), a chemical modifier of carboxylic amino acid residues. Modification of the COOH groups inhibits the light-induced oxidation of exogenous Mn(II) cations by Mn-depleted photosystem II (PSII[-Mn]) membranes. The rate of Mn(II) oxidation saturates at ≥10 μM in PSII(-Mn) membranes and ≥500 μM in EDC-treated PSII (-Mn) samples. Intact PSII(-Mn) membranes have only one site for Mn(II) oxidation via YZ (dissociation constant, Kd = 0.64 μM), while EDC-treated PSII(-Mn) samples have two sites (Kd = 1.52 and 22 μM; the latter is the low-affinity site). When PSII(-Mn) membranes were incubated with Fe(II) before modifier treatment (to block the high-affinity site) and the blocking iron cations were extracted with citrate (pH 3.0) after modification, the membranes contained only one site (Kd = 2.3 μM) for exogenous Mn(II) oxidation by YZ{radical dot} radical. In this case, the rate of electron donation via YZ saturated at a Mn(II) concentration ≥15 μM. These results indicate that the carboxylic residue participating in Mn(II) coordination and the binding of oxidized manganese cations at the HAZ site is protected from the action of the modifier by the iron cations blocking the HAZ site. We concluded that the carboxylic residue (D1 Asp-170) participating in the coordination of the manganese cation at the HAZ site (Mn4 in the tetranuclear manganese cluster [Science 303 (2004) 1831]) is also involved in the ligation of the Fe cation(s) blocking the high-affinity Mn-binding site.

Original languageAmerican English
Pages (from-to)189-197
Number of pages9
JournalBBA - Bioenergetics
Volume1757
Issue number3
DOIs
StatePublished - 2006

NREL Publication Number

  • NREL/JA-590-39483

Keywords

  • Carboxylic amino acid
  • Chemical modification
  • Citrate
  • D1 Asp-170
  • High-affinity site
  • Iron
  • Low affinity site
  • Low pH
  • Manganese
  • Metal ion binding
  • Metal ion oxidation
  • Photosystem II
  • PSII
  • Y

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