Cellulose Hydrolysis by Clostridium thermocellum is Agnostic to Substrate Structural Properties in Contrast to Fungal Cellulases

Ashutosh Mittal, Michael Himmel, Ninad Kothari, Samarthya Bhagia, Maher Zaher, Yunqiao Pu, Chang Yoo, Arthur Ragauskas, Rajeev Kumar, Charles Wyman

Research output: Contribution to journalArticlepeer-review

11 Scopus Citations

Abstract

The native recalcitrance of lignocellulosic biomass hinders its effective deconstruction for biological conversion to fuel ethanol. However, once cellulose is physically available to enzymes/microbes, i.e., macro-accessible, cellulose micro-accessibility, i.e., the accessibility as influenced by cellulose properties, further affects cellulose conversion. Here, we performed a comparative study of the effect of cellulose micro-accessibility on cellulose conversion by two biological approaches of potential commercial interest: Consolidated bioprocessing (CBP) using Clostridium thermocellum and cell-free saccharification mediated by fungal enzymes. Commercially available cellulosic substrates, Avicel® PH-101, Sigmacell Cellulose Type 50, cotton linters, Whatman™ 1 milled filter paper, and α-cellulose were employed to constitute different cellulose micro-accessibilities. Physiochemical characterization was performed on these substrates to determine key morphological and chemical differences. Biological conversion of these substrates showed that C. thermocellum was unaffected overall by cellulose structural properties, i.e., micro-accessibility, and achieved similar solids solubilization and metabolite production from these structurally different materials. However, fungal enzymes digested these substrates to different extents. Specifically, glucan conversion of these substrates diminished in the following order: Milled filter paper > Avicel > Sigmacell and α-cellulose > cotton linters. Here, we propose that C. thermocellum digestion of lignocellulosic biomass is primarily controlled by the physical availability of cellulose in the lignocellulosic matrix and largely unaffected by cellulose properties once cellulose is made macro-accessible. In contrast, fungal enzymes require cellulose to be physically accessible, i.e., macro-accessible, as well as have properties amenable to digestion, i.e., micro-accessible.

Original languageAmerican English
Pages (from-to)2810-2822
Number of pages13
JournalGreen Chemistry
Volume21
Issue number10
DOIs
StatePublished - 2019

Bibliographical note

Publisher Copyright:
© 2019 The Royal Society of Chemistry.

NREL Publication Number

  • NREL/JA-2700-74123

Keywords

  • cellulose conversion
  • consolidated bioprocessing
  • fungal enzymes
  • lignocellulosic biomass
  • saccharification

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