Chapter 16: Lignin Visualization: Advanced Microscopy Techniques for Lignin Characterization

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3 Scopus Citations


Visualization of lignin in plant cell walls, with both spatial and chemical resolution, is emerging as an important tool to understand lignin's role in the plant cell wall's nanoscale architecture and to understand and design processes intended to modify the lignin. As such, this chapter reviews recent advances in advanced imaging methods with respect to lignin in plant cell walls. This review focuses on the importance of lignin detection and localization for studies in both plant biology and biotechnology. Challenges going forward to identify and delineate lignin from other plant cell wall components and to quantitatively analyze lignin in whole cell walls from native plant tissue and treated biomass are also discussed.

Original languageAmerican English
Title of host publicationLignin Valorization: Emerging Approaches
Subtitle of host publicationRSC Energy and Environment Series, No. 19
EditorsGregg T. Beckham
PublisherRoyal Society of Chemistry
Number of pages22
ISBN (Electronic)9781782620426, 9781782625544, 9781782629252
StatePublished - 2018

Publication series

NameRSC Energy and Environment Series
ISSN (Print)2044-0774
ISSN (Electronic)2044-0782

Bibliographical note

Publisher Copyright:
© The Royal Society of Chemistry 2018.

NREL Publication Number

  • NREL/CH-2700-71435


  • detection
  • imaging methods
  • lignin
  • localization
  • nanoscale architecture


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