Chapter 2: Genetics of Unstudied Thermophiles for Industry

Daehwan Chung; Nicholas Sarai; Michael Himmel; Yannick Bomble

doi:10.1007/978-1-0716-0195-2_2

Chapter 2: Genetics of Unstudied Thermophiles for Industry

Daehwan Chung, Nicholas Sarai, Michael Himmel, Yannick Bomble

California Institute of Technology

Research output: Chapter in Book/Report/Conference proceeding › Chapter › peer-review

2 Scopus Citations

Abstract

Thermophilic organisms hold great potential for industry due to their numerous advantages in biotechnological applications such as higher reaction rate, higher substrate loading, decreased susceptibility to reaction contamination, energy savings in industrial fermentations, and ability to express thermostable proteins that can be utilized in many important industrial processes. Bioprospecting for thermophiles will continue to reveal new enzymatic and metabolic paradigms with industrial applicability. In order to translate these paradigms to production scale, routine methods for microbial genetic engineering are needed, yet remain to be developed in many newly isolated thermophiles. Major challenges and recent developments in the establishment of reliable genetic systems in thermophiles are discussed. Here, we use a hyperthermophilic, cellulolytic bacterium, Caldicellulosiruptor bescii, as a case study to demonstrate the development of a genetic system for an industrially useful thermophile, describing in detail methods for transformation, genetic tool utilization, and chromosomal modification using targeted gene deletion and insertion techniques.

Original language	American English
Title of host publication	Metabolic Pathway Engineering
Subtitle of host publication	Methods in Molecular Biology, Volume 2096
Editors	M. E. Himmel, Y. J. Bomble
Publisher	Humana Press Inc.
Pages	5-19
Number of pages	15
DOIs	https://doi.org/10.1007/978-1-0716-0195-2_2 https://doi.org/10.1007/978-1-0716-0195-2_2
State	Published - 2020

Publication series

Name	Methods in Molecular Biology
Volume	2096
ISSN (Print)	1064-3745
ISSN (Electronic)	1940-6029

Bibliographical note

Publisher Copyright:
© Springer Science+Business Media, LLC, part of Springer Nature 2020.

NREL Publication Number

NREL/CH-2700-74950

Keywords

Biofuels
Caldicellulosiruptor bescii
Electroporation
Genetic engineering
Restriction-modification system
Thermophiles
Thermostable proteins

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Chung, D., Sarai, N., Himmel, M., & Bomble, Y. (2020). Chapter 2: Genetics of Unstudied Thermophiles for Industry. In M. E. Himmel, & Y. J. Bomble (Eds.), Metabolic Pathway Engineering: Methods in Molecular Biology, Volume 2096 (pp. 5-19). (Methods in Molecular Biology; Vol. 2096). Humana Press Inc.. https://doi.org/10.1007/978-1-0716-0195-2_2, https://doi.org/10.1007/978-1-0716-0195-2_2

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Chung, D, Sarai, N, Himmel, M & Bomble, Y 2020, Chapter 2: Genetics of Unstudied Thermophiles for Industry. in ME Himmel & YJ Bomble (eds), Metabolic Pathway Engineering: Methods in Molecular Biology, Volume 2096. Methods in Molecular Biology, vol. 2096, Humana Press Inc., pp. 5-19. https://doi.org/10.1007/978-1-0716-0195-2_2, https://doi.org/10.1007/978-1-0716-0195-2_2

Chapter 2: Genetics of Unstudied Thermophiles for Industry. / Chung, Daehwan; Sarai, Nicholas; Himmel, Michael et al.
Metabolic Pathway Engineering: Methods in Molecular Biology, Volume 2096. ed. / M. E. Himmel; Y. J. Bomble. Humana Press Inc., 2020. p. 5-19 (Methods in Molecular Biology; Vol. 2096).

Research output: Chapter in Book/Report/Conference proceeding › Chapter › peer-review

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PB - Humana Press Inc.

ER -

Chapter 2: Genetics of Unstudied Thermophiles for Industry

Abstract

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Keywords

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