Chapter 20: Advances and Application of CRISPR-Cas Systems

Rongming Liu, Liya Liang, Margaret Habib, Emily Freed, Carrie Eckert

Research output: Chapter in Book/Report/Conference proceedingChapter


A new gene-editing technique called clustered regularly interspaced short palindromic repeats (CRISPR) and CRISPR-associated (Cas) proteins have revolutionized genome engineering because of its high efficiency, relatively low cost, and ease of use compared with other techniques such as zinc-finger nucleases and transcription activator-like effector nucleases. CRISPR-Cas systems have transformed biological research, quickly becoming the preferred method for engineering specific genome sequences in industrially relevant microbes, important food crops, and human cells. This powerful new tool has the potential for limitless applications ranging from the treatment of human diseases to improving food security to the generation of renewable and sustainable bioproducts and biofuels. In this chapter, we will discuss advances and applications for these CRISPR-Cas systems in biological engineering.
Original languageAmerican English
Title of host publicationNew Frontiers and Applications of Synthetic Biology
EditorsV. Singh
StatePublished - 2022

NREL Publication Number

  • NREL/CH-2700-78846


  • bacterial
  • CRISPR-Cas
  • gene regulation
  • genome editing
  • genome engineering
  • human cells
  • metabolic engineering
  • plant
  • yeast


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