Chapter 20: Advances and Application of CRISPR-Cas Systems

Rongming Liu; Liya Liang; Margaret Habib; Emily Freed; Carrie Eckert

doi:10.1016/C2020-0-01945-6

Chapter 20: Advances and Application of CRISPR-Cas Systems

Rongming Liu, Liya Liang, Margaret Habib, Emily Freed, Carrie Eckert

Biosciences Center

University of Colorado Boulder

Research output: Chapter in Book/Report/Conference proceeding › Chapter

Abstract

A new gene-editing technique called clustered regularly interspaced short palindromic repeats (CRISPR) and CRISPR-associated (Cas) proteins have revolutionized genome engineering because of its high efficiency, relatively low cost, and ease of use compared with other techniques such as zinc-finger nucleases and transcription activator-like effector nucleases. CRISPR-Cas systems have transformed biological research, quickly becoming the preferred method for engineering specific genome sequences in industrially relevant microbes, important food crops, and human cells. This powerful new tool has the potential for limitless applications ranging from the treatment of human diseases to improving food security to the generation of renewable and sustainable bioproducts and biofuels. In this chapter, we will discuss advances and applications for these CRISPR-Cas systems in biological engineering.

Original language	American English
Title of host publication	New Frontiers and Applications of Synthetic Biology
Editors	V. Singh
Pages	331-348
DOIs	https://doi.org/10.1016/C2020-0-01945-6
State	Published - 2022

NREL Publication Number

NREL/CH-2700-78846

Keywords

bacterial
CRISPR
CRISPR-Cas
gene regulation
genome editing
genome engineering
human cells
metabolic engineering
plant
yeast

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10.1016/C2020-0-01945-6

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abstract = "A new gene-editing technique called clustered regularly interspaced short palindromic repeats (CRISPR) and CRISPR-associated (Cas) proteins have revolutionized genome engineering because of its high efficiency, relatively low cost, and ease of use compared with other techniques such as zinc-finger nucleases and transcription activator-like effector nucleases. CRISPR-Cas systems have transformed biological research, quickly becoming the preferred method for engineering specific genome sequences in industrially relevant microbes, important food crops, and human cells. This powerful new tool has the potential for limitless applications ranging from the treatment of human diseases to improving food security to the generation of renewable and sustainable bioproducts and biofuels. In this chapter, we will discuss advances and applications for these CRISPR-Cas systems in biological engineering.",

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AU - Freed, Emily

AU - Eckert, Carrie

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AB - A new gene-editing technique called clustered regularly interspaced short palindromic repeats (CRISPR) and CRISPR-associated (Cas) proteins have revolutionized genome engineering because of its high efficiency, relatively low cost, and ease of use compared with other techniques such as zinc-finger nucleases and transcription activator-like effector nucleases. CRISPR-Cas systems have transformed biological research, quickly becoming the preferred method for engineering specific genome sequences in industrially relevant microbes, important food crops, and human cells. This powerful new tool has the potential for limitless applications ranging from the treatment of human diseases to improving food security to the generation of renewable and sustainable bioproducts and biofuels. In this chapter, we will discuss advances and applications for these CRISPR-Cas systems in biological engineering.

KW - bacterial

KW - CRISPR

KW - CRISPR-Cas

KW - gene regulation

KW - genome editing

KW - genome engineering

KW - human cells

KW - metabolic engineering

KW - plant

KW - yeast

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BT - New Frontiers and Applications of Synthetic Biology

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ER -

Chapter 20: Advances and Application of CRISPR-Cas Systems

Abstract

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Keywords

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