Chapter 3: Isolation of Photosystem II Reaction Center Complexes from Plants

Michael Seibert, Rafael Picorel

Research output: Chapter in Book/Report/Conference proceedingChapterpeer-review

3 Scopus Citations


Methods to isolate and purify 6- and 5-Chl D1/D2/Cyt b559 photosystem II (PSII) reaction center (RC) complexes from plants are presented, and the advantages and disadvantages of each procedure are discussed. One of the simpler 6-Chl procedures and a procedure for isolating 5-Chl complexes are described in detail. Furthermore, a rapid procedure that produces relatively large amounts of less pure 6-Chl material (i.e., more nonpigmented protein) is also described. Criteria to assess the purity of PSII RC preparations are presented, and problems associated with each of the isolation procedures are discussed.

Original languageAmerican English
Title of host publicationPhotosynthesis Research Protocols
Subtitle of host publicationMethods in Molecular Biology, Vol. 684
EditorsR. Carpentier
PublisherHumana Press Inc.
Number of pages11
StatePublished - 2011

Publication series

NameMethods in Molecular Biology
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029

Bibliographical note

Publisher Copyright:
© 2011, Springer Science+Business Media, LLC.

NREL Publication Number

  • NREL/CH-2A00-52958


  • Chromatography
  • Intrinsic protein
  • Isolation
  • Photosystem II
  • Pigment-protein complex
  • Purification
  • Reaction center


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