Chapter 3: Isolation of Photosystem II Reaction Center Complexes from Plants

Michael Seibert; Rafael Picorel

doi:10.1007/978-1-60761-925-3_3

Chapter 3: Isolation of Photosystem II Reaction Center Complexes from Plants

Michael Seibert, Rafael Picorel

BioEconomy and Sustainable Transportation

Estacion Experimental de Aula Dei (CSIC)

Research output: Chapter in Book/Report/Conference proceeding › Chapter › peer-review

3 Scopus Citations

Abstract

Methods to isolate and purify 6- and 5-Chl D1/D2/Cyt b₅₅₉ photosystem II (PSII) reaction center (RC) complexes from plants are presented, and the advantages and disadvantages of each procedure are discussed. One of the simpler 6-Chl procedures and a procedure for isolating 5-Chl complexes are described in detail. Furthermore, a rapid procedure that produces relatively large amounts of less pure 6-Chl material (i.e., more nonpigmented protein) is also described. Criteria to assess the purity of PSII RC preparations are presented, and problems associated with each of the isolation procedures are discussed.

Original language	American English
Title of host publication	Photosynthesis Research Protocols
Subtitle of host publication	Methods in Molecular Biology, Vol. 684
Editors	R. Carpentier
Publisher	Humana Press Inc.
Pages	17-27
Number of pages	11
DOIs	https://doi.org/10.1007/978-1-60761-925-3_3 https://doi.org/10.1007/978-1-60761-925-3_3
State	Published - 2011

Publication series

Name	Methods in Molecular Biology
Volume	684
ISSN (Print)	1064-3745
ISSN (Electronic)	1940-6029

Bibliographical note

Publisher Copyright:
© 2011, Springer Science+Business Media, LLC.

NREL Publication Number

NREL/CH-2A00-52958

Keywords

Chromatography
Intrinsic protein
Isolation
Photosystem II
Pigment-protein complex
Purification
Reaction center

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Seibert, M., & Picorel, R. (2011). Chapter 3: Isolation of Photosystem II Reaction Center Complexes from Plants. In R. Carpentier (Ed.), Photosynthesis Research Protocols: Methods in Molecular Biology, Vol. 684 (pp. 17-27). (Methods in Molecular Biology; Vol. 684). Humana Press Inc.. https://doi.org/10.1007/978-1-60761-925-3_3, https://doi.org/10.1007/978-1-60761-925-3_3

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abstract = "Methods to isolate and purify 6- and 5-Chl D1/D2/Cyt b559 photosystem II (PSII) reaction center (RC) complexes from plants are presented, and the advantages and disadvantages of each procedure are discussed. One of the simpler 6-Chl procedures and a procedure for isolating 5-Chl complexes are described in detail. Furthermore, a rapid procedure that produces relatively large amounts of less pure 6-Chl material (i.e., more nonpigmented protein) is also described. Criteria to assess the purity of PSII RC preparations are presented, and problems associated with each of the isolation procedures are discussed.",

keywords = "Chromatography, Intrinsic protein, Isolation, Photosystem II, Pigment-protein complex, Purification, Reaction center",

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year = "2011",

doi = "10.1007/978-1-60761-925-3_3",

language = "American English",

series = "Methods in Molecular Biology",

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Chapter 3: Isolation of Photosystem II Reaction Center Complexes from Plants. / Seibert, Michael; Picorel, Rafael.
Photosynthesis Research Protocols: Methods in Molecular Biology, Vol. 684. ed. / R. Carpentier. Humana Press Inc., 2011. p. 17-27 (Methods in Molecular Biology; Vol. 684).

Research output: Chapter in Book/Report/Conference proceeding › Chapter › peer-review

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AB - Methods to isolate and purify 6- and 5-Chl D1/D2/Cyt b559 photosystem II (PSII) reaction center (RC) complexes from plants are presented, and the advantages and disadvantages of each procedure are discussed. One of the simpler 6-Chl procedures and a procedure for isolating 5-Chl complexes are described in detail. Furthermore, a rapid procedure that produces relatively large amounts of less pure 6-Chl material (i.e., more nonpigmented protein) is also described. Criteria to assess the purity of PSII RC preparations are presented, and problems associated with each of the isolation procedures are discussed.

KW - Chromatography

KW - Intrinsic protein

KW - Isolation

KW - Photosystem II

KW - Pigment-protein complex

KW - Purification

KW - Reaction center

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Chapter 3: Isolation of Photosystem II Reaction Center Complexes from Plants

Abstract

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Keywords

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