Chapter 4: Methods for Metabolic Engineering of a Filamentous Trichoderma reesei

Yat-Chen Chou; Arjun Singh; Qi Xu; Michael Himmel; Min Zhang

doi:10.1007/978-1-0716-0195-2_4

Chapter 4: Methods for Metabolic Engineering of a Filamentous Trichoderma reesei

Yat-Chen Chou, Arjun Singh, Qi Xu, Michael Himmel, Min Zhang

National Renewable Energy Laboratory

Research output: Chapter in Book/Report/Conference proceeding › Chapter › peer-review

Abstract

In this work, we describe genetic tools and techniques for engineering Trichoderma reesei for the production of farnesene. Enhanced production of farnesene was used as an example of this methodology; as were the overexpression of a key enzyme, HMGS, in the MVA pathway.

Original language	American English
Title of host publication	Metabolic Pathway Engineering
Subtitle of host publication	Methods in Molecular Biology, Volume 2096
Editors	M. E. Himmel, Y. J. Bomble
Publisher	Humana Press Inc.
Pages	45-50
Number of pages	6
DOIs	https://doi.org/10.1007/978-1-0716-0195-2_4 https://doi.org/10.1007/978-1-0716-0195-2_4
State	Published - 2020

Publication series

Name	Methods in Molecular Biology
Volume	2096
ISSN (Print)	1064-3745
ISSN (Electronic)	1940-6029

Bibliographical note

Publisher Copyright:
© Springer Science+Business Media, LLC, part of Springer Nature 2020.

NREL Publication Number

NREL/CH-2700-74953

Keywords

Direct microbial conversion
Trichoderma reesei
β-Farnesene

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Chou, Y.-C., Singh, A., Xu, Q., Himmel, M., & Zhang, M. (2020). Chapter 4: Methods for Metabolic Engineering of a Filamentous Trichoderma reesei. In M. E. Himmel, & Y. J. Bomble (Eds.), Metabolic Pathway Engineering: Methods in Molecular Biology, Volume 2096 (pp. 45-50). (Methods in Molecular Biology; Vol. 2096). Humana Press Inc.. https://doi.org/10.1007/978-1-0716-0195-2_4, https://doi.org/10.1007/978-1-0716-0195-2_4

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Chou, Y-C, Singh, A, Xu, Q , Himmel, M & Zhang, M 2020, Chapter 4: Methods for Metabolic Engineering of a Filamentous Trichoderma reesei. in ME Himmel & YJ Bomble (eds), Metabolic Pathway Engineering: Methods in Molecular Biology, Volume 2096. Methods in Molecular Biology, vol. 2096, Humana Press Inc., pp. 45-50. https://doi.org/10.1007/978-1-0716-0195-2_4, https://doi.org/10.1007/978-1-0716-0195-2_4

Chapter 4: Methods for Metabolic Engineering of a Filamentous Trichoderma reesei. / Chou, Yat-Chen; Singh, Arjun; Xu, Qi et al.
Metabolic Pathway Engineering: Methods in Molecular Biology, Volume 2096. ed. / M. E. Himmel; Y. J. Bomble. Humana Press Inc., 2020. p. 45-50 (Methods in Molecular Biology; Vol. 2096).

Research output: Chapter in Book/Report/Conference proceeding › Chapter › peer-review

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Chapter 4: Methods for Metabolic Engineering of a Filamentous Trichoderma reesei

Abstract

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Keywords

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