Chapter 4: Methods for Metabolic Engineering of a Filamentous Trichoderma reesei

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Abstract

In this work, we describe genetic tools and techniques for engineering Trichoderma reesei for the production of farnesene. Enhanced production of farnesene was used as an example of this methodology; as were the overexpression of a key enzyme, HMGS, in the MVA pathway.

Original languageAmerican English
Title of host publicationMetabolic Pathway Engineering
Subtitle of host publicationMethods in Molecular Biology, Volume 2096
EditorsM. E. Himmel, Y. J. Bomble
PublisherHumana Press Inc.
Pages45-50
Number of pages6
DOIs
StatePublished - 2020

Publication series

NameMethods in Molecular Biology
Volume2096
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029

Bibliographical note

Publisher Copyright:
© Springer Science+Business Media, LLC, part of Springer Nature 2020.

NREL Publication Number

  • NREL/CH-2700-74953

Keywords

  • Direct microbial conversion
  • Trichoderma reesei
  • β-Farnesene

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