Chapter 5: Meso-Scale Modeling of Polysaccharides in Plant Cell Walls: An Application to Translation of CBMs on Cellulose Surface

Research output: Chapter in Book/Report/Conference proceedingChapterpeer-review

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Abstract

A coarse-grained model and force field for simulating cellulose Iβ surface (1,0,0) was derived, in which each β-D-glucose unit is represented by three beads. The coarse-grained model can reproduce a stable cellulose (1,0,0) surface with an excellent agreement with an all-atom model. When used to study the interaction of the family 1 carbohydrate-binding module (CBM1) with this cellulose surface model, the CBM "opens" as in earlier atomistic simulations. This cellulose Iβ surface model produces simulations in which the CBM translates along a broken cellodextrin chain. This processive motion of the exoglucanase cellobiohydrolase I has long been suggested by experimental studies, but has never before been observed in computer simulations.

Original languageAmerican English
Title of host publicationComputational Modeling in Lignocellulosic Biofuel Production
Subtitle of host publicationACS Symposium Series, Vol. 1052
PublisherAmerican Chemical Society
Pages99-117
Number of pages19
ISBN (Print)9780841225718
DOIs
StatePublished - 14 Dec 2010

Publication series

NameACS Symposium Series
Volume1052
ISSN (Print)0097-6156
ISSN (Electronic)1947-5918

NREL Publication Number

  • NREL/CH-510-48873

Keywords

  • biomass
  • cellobiohydrolase
  • cellulose
  • polysaccharides

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