Chapter 5: Methods for Algal Protein Isolation and Proteome Analysis

Eric Knoshaug, Alida Gerritsen, Calvin Henard, Michael Guarnieri

Research output: Chapter in Book/Report/Conference proceedingChapterpeer-review

1 Scopus Citations


Microalgae present promising feedstocks to produce renewable fuel and chemical intermediates, in part due to high storage carbon flux capacity to triacylglycerides or storage carbohydrates upon nutrient deprivation. However, the mechanism(s) governing deprivation-mediated carbon partitioning remain to be fully elucidated, limiting targeted strain engineering strategies in algal biocatalysts. Though genomic and transcriptomic analyses offer key insights into these mechanisms, active post-transcriptional regulatory mechanisms, ubiquitous in many microalgae, necessitate proteomic and post-translational (e.g., phospho- and nitroso-proteomic) analyses to more completely evaluate algal responsiveness to nutrient deprivation. Herein, we describe methods for isolating total algal protein and conducting proteomic, phosphoproteomic, and nitrosoproteomic analyses. We focus on methods deployed for the chlorophyte, Chlorella vulgaris, a model oleaginous alga with high flux to renewable fuel and chemical precursors.

Original languageAmerican English
Title of host publicationMetabolic Pathway Engineering
Subtitle of host publicationMethods in Molecular Biology, Volume 2096
EditorsM. E. Himmel, Y. J. Bomble
PublisherHumana Press Inc.
Number of pages9
StatePublished - 2020

Publication series

NameMethods in Molecular Biology
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029

Bibliographical note

Publisher Copyright:
© Springer Science+Business Media, LLC, part of Springer Nature 2020.

NREL Publication Number

  • NREL/CH-2700-77587


  • Biofuels
  • Chlorella vulgaris
  • Microalgae
  • Proteomics


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