Abstract
Microalgae present promising feedstocks to produce renewable fuel and chemical intermediates, in part due to high storage carbon flux capacity to triacylglycerides or storage carbohydrates upon nutrient deprivation. However, the mechanism(s) governing deprivation-mediated carbon partitioning remain to be fully elucidated, limiting targeted strain engineering strategies in algal biocatalysts. Though genomic and transcriptomic analyses offer key insights into these mechanisms, active post-transcriptional regulatory mechanisms, ubiquitous in many microalgae, necessitate proteomic and post-translational (e.g., phospho- and nitroso-proteomic) analyses to more completely evaluate algal responsiveness to nutrient deprivation. Herein, we describe methods for isolating total algal protein and conducting proteomic, phosphoproteomic, and nitrosoproteomic analyses. We focus on methods deployed for the chlorophyte, Chlorella vulgaris, a model oleaginous alga with high flux to renewable fuel and chemical precursors.
Original language | American English |
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Title of host publication | Metabolic Pathway Engineering |
Subtitle of host publication | Methods in Molecular Biology, Volume 2096 |
Editors | M. E. Himmel, Y. J. Bomble |
Publisher | Humana Press Inc. |
Pages | 51-59 |
Number of pages | 9 |
DOIs | |
State | Published - 2020 |
Publication series
Name | Methods in Molecular Biology |
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Volume | 2096 |
ISSN (Print) | 1064-3745 |
ISSN (Electronic) | 1940-6029 |
Bibliographical note
Publisher Copyright:© Springer Science+Business Media, LLC, part of Springer Nature 2020.
NREL Publication Number
- NREL/CH-2700-77587
Keywords
- Biofuels
- Chlorella vulgaris
- Microalgae
- Proteomics