Chapter 7 - CRISPR-Based Tools for Microbial Cell Factories

Rongming Liu, Liya Liang, Sean Stettner, Emily Freed, Carrie Eckert

Research output: Chapter in Book/Report/Conference proceedingChapter


The development of microbial chassis for the production of a variety of biochemicals and biofuels is a growing area of research. How to efficiently manipulate genetic information to achieve optimal production of these compounds is a key area of focus in the field. In recent years, clustered regularly interspaced palindromic repeats (CRISPR) and its associated proteins (Cas) have become a popular strategy for gene editing and regulation in many organisms due to its versatility and efficacy. Here, we describe methods developed utilizing CRISPR-Cas systems for engineering microbial cell factories (e.g., bacteria and yeast) at the single gene to genome scale for mutagenesis and transcriptional regulation of target genes. Finally, we provide a perspective on the challenges and opportunities for the applications of advanced CRISPR-Cas-based tools for engineering microbial cell factories.
Original languageAmerican English
Title of host publicationMicrobial Cell Factories Engineering for Production of Biomolecules
EditorsV. Singh
StatePublished - 2021

NREL Publication Number

  • NREL/CH-2700-76795


  • CRISPR-Cas
  • gene editing
  • genome editing


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