Characterization of Photosynthetic Reaction Centers by Surface-Enhanced Resonance Raman Scattering

George Chumanov, Therese M. Cotton, Chengli Zhou, Dale Gaul, Rafael Picorel, Michael Seibert

Research output: Contribution to conferencePaperpeer-review


Surface-enhanced Resonance Raman scattering (SERRS) spectra were obtained for the reaction center complexes of the photosynthetic bacterium Rhodobacter sphaeroides (RC) and from photosystem II (PSII) of spinach, adsorbed on Ag and Au surfaces. These preliminary results demonstrate the considerable potential of this technique for selectively exciting resonance Raman scattering from reaction center components within their distinct absorption bands. Because of the high sensitivity afforded by SERRS, spectra could be measured from a single monolayer of reaction centers adsorbed on a metal surface. The surface-sensitivity provides new information indicating the topology of the PSII reaction center 47 kD light-harvesting protein complex. The activity of the PS II reaction center complex adsorbed on metal surfaces was monitored by photochemical reduction of cyt b-559. Measurement of fluorescence emission was shown to be a new and sensitive method for monitoring the structural and functional integrity of the PS II reaction center complex on the metal surface.

Original languageAmerican English
Number of pages9
StatePublished - 17 Jun 1993
EventLaser Spectroscopy of Biomolecules 1992 - Jyvaskyla, Finland
Duration: 7 Sep 199211 Sep 1992


ConferenceLaser Spectroscopy of Biomolecules 1992

Bibliographical note

Publisher Copyright:
© 1993 SPIE. All rights reserved.

NREL Publication Number

  • ACNR/CP-16353


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