Chemical, Ultrastructural and Supramolecular Analysis of Tension Wood in Populus Tremula x Alba as a Model Substrate for Reduced Recalcitrance

Marcus Foston, Christopher A. Hubbell, Reichel Samuel, Seokwon Jung, Hu Fan, Shi You Ding, Yining Zeng, Sara Jawdy, Mark Davis, Robert Sykes, Erica Gjersing, Gerald A. Tuskan, Udaya Kalluri, Arthur J. Ragauskas

Research output: Contribution to journalArticlepeer-review

58 Scopus Citations

Abstract

Biomass is one of the most abundant potential sustainable sources for fuel and material production, however to fully realize this potential an improved understanding of lignocellulosic recalcitrance must be developed. In an effort to appreciate the underlying phenotypic, biochemical and morphological properties associated with the reduced recalcitrance observed in tension stress-induced reaction wood, we report the increased enzymatic sugar yield and corresponding chemical and ultrastructural properties of Populus tension wood. Populus tremula x alba (PTA) was grown under tension and stem segments containing three different wood types: normal wood (NW), tension wood (TW) from the elongated stem side and opposite wood (OW) from the compressed stem side were collected. A variety of analytical techniques were used to describe changes occurring as a result of the tension stress-induced formation of a gelatinous cell wall layer (G-layer). For example, gel permeation chromatography (GPC) and 13C solid-state nuclear magnetic resonance (NMR) revealed that the molecular weight and crystallinity of cellulose in TW is greater than that of cellulose acquired from NW. Whole cell ionic liquid and other solid-state NMR analysis detailed the structure of lignin and hemicellulose in the samples, detecting the presence of variations in lignin and hemicellulose sub-units, linkages and semi-quantitatively estimating the relative amounts of syringyl (S), guaiacyl (G) and p-hydroxybenzoate (PB) monolignol units. It was confirmed that TW displayed an increase in PB or H-like lignin and S to G ratio from 1.25 to 1.50 when compared to the NW sample. Scanning electron microscopy (SEM) and coherent anti-Stokes Raman scattering (CARS) were also used to evaluate the morphology and corresponding spatial distribution of the major lignocellulosic components. We found changes in a combination of cell wall properties appear to influence recalcitrance more than any single factor alone.

Original languageAmerican English
Pages (from-to)4962-4971
Number of pages10
JournalEnergy and Environmental Science
Volume4
Issue number12
DOIs
StatePublished - 2011

NREL Publication Number

  • NREL/JA-2700-53783

Keywords

  • lignocellulosic recalcitrance
  • tension wood

Fingerprint

Dive into the research topics of 'Chemical, Ultrastructural and Supramolecular Analysis of Tension Wood in Populus Tremula x Alba as a Model Substrate for Reduced Recalcitrance'. Together they form a unique fingerprint.

Cite this