Coherent Raman Microscopy Analysis of Plant Cell Walls

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17 Scopus Citations


Coherent Raman scattering (CRS) microscopy is a label-free method for chemical imaging, as it offers chemical specificity with orders of magnitude better sensitivity than the state-of-the-art confocal Raman scattering microscopy. Currently CRS technique includes coherent anti-Stokes Raman scattering (CARS), and stimulated Raman scattering (SRS). This chapter describes the methods of using CRS microscopy to image major polymers in plant cell wall (i.e., lignin and cellulose). This method can also be used to real-time monitor the chemical processes involved in biomass pretreatment. These together demonstrate CRS as an effective method for imaging complex chemistry in biological systems.

Original languageAmerican English
Title of host publicationBiomass Conversion
Subtitle of host publicationMethods and Protocols
EditorsMichael Himmel
Number of pages12
StatePublished - 2012

Publication series

NameMethods in Molecular Biology
ISSN (Print)1064-3745

NREL Publication Number

  • NREL/CH-2700-52559


  • Biofuels
  • Cellulose
  • Coherent anti-Stokes Raman scattering miscroscopy
  • Coherent Raman scattering
  • Lignin
  • Plant cell wall
  • Raman spectroscopy
  • Stimulated Raman scattering microscopy


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