Comparison of Biomass and Lipid Production under Ambient Carbon Dioxide Vigorous Aeration and 3% Carbon Dioxide Condition Among the Lead Candidate Chlorella Strains Screened by Various Photobioreactor Scales

Michael Guarnieri, Naoko Kobayashi, Austin Barnes, Travis Jensen, Eric Noel, Gunjan Andlay, Julian Rosenberg, Michael Betenbaugh, George Oyler

Research output: Contribution to journalArticlepeer-review

12 Scopus Citations

Abstract

Chlorella species from the UTEX collection, classified by rDNA-based phylogenetic analysis, were screened based on biomass and lipid production in different scales and modes of culture. The lead candidate strains of C. sorokiniana UTEX 1230 and C. vulgaris UTEX 395 and 259 were compared between conditions of vigorous aeration with filtered atmospheric air and 3% CO2 shake-flask cultivation. The biomass of UTEX 1230 produced 2 times higher at 652mgL-1 dry weight under both ambient CO2 vigorous aeration and 3% CO2 conditions, while UTEX 395 and 259 under 3% CO2 increased to 3 times higher at 863mgL-1 dry weight than ambient CO2 vigorous aeration. The triacylglycerol contents of UTEX 395 and 259 increased more than 30 times to 30% dry weight with 3% CO2, indicating that additional CO2 is essential for both biomass and lipid accumulation in UTEX 395 and 259.

Original languageAmerican English
Pages (from-to)246-255
Number of pages10
JournalBioresource Technology
Volume198
DOIs
StatePublished - 2015

Bibliographical note

Publisher Copyright:
© 2015.

NREL Publication Number

  • NREL/JA-5100-65216

Keywords

  • Biomass
  • Chlorella sorokiniana
  • Chlorella vulgaris
  • Lipid content
  • RDNA-based phylogenetic tree

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