Abstract
An aspect of the present disclosure is a microbial cell that includes a genetic modification resulting in the expression of a deficient form of an endogenous dioxygenase, and a gene encoding an exogenous dioxygenase and a promoter sequence, where the endogenous dioxygenase includes PcaH and PcaG, the exogenous dioxygenase includes LigA and LigB, the microbial cell is capable of growth utilizing at least one of a cellulose decomposition molecule or a lignin decomposition molecule, and the microbial cell is capable of producing 2-hydroxy-2H-pyran-4,6-dicarboxylic acid.
| Original language | American English |
|---|---|
| Patent number | US 10,253,338 B2 |
| Filing date | 9/04/19 |
| State | Published - 2019 |
NREL Publication Number
- NREL/PT-2A00-73706
Keywords
- biomass
- cellulose decomposition
- exogenous dioxygenase
- gene encoding
- lignin
- promoter sequence