Abstract
An aspect of the present disclosure is a microbial cell that includes a genetic modification resulting in the expression of a deficient form of an endogenous dioxygenase, and a gene encoding an exogenous dioxygenase and a promoter sequence, where the endogenous dioxygenase includes PcaH and PcaG, the exogenous dioxygenase includes LigA and LigB, the microbial cell is capable of growth utilizing at least one of a cellulose decomposition molecule or a lignin decomposition molecule, and the microbial cell is capable of producing 2-hydroxy-2H-pyran-4,6-dicarboxylic acid.
Original language | American English |
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Patent number | 11,326,190 B2 |
Filing date | 10/05/22 |
State | Published - 2022 |
NREL Publication Number
- NREL/PT-2A00-82927
Keywords
- amino acid sequence
- endogenous dioxygenase
- lignocellulose
- nucleic acid sequence