Conversion of Biomass to Useful Intermediates

Gregg Beckham (Inventor), Christopher Johnson (Inventor), Derek Vardon (Inventor)

Research output: Patent

Abstract

An aspect of the present disclosure is a microbial cell that includes a genetic modification resulting in the expression of a deficient form of an endogenous dioxygenase, and a gene encoding an exogenous dioxygenase and a promoter sequence, where the endogenous dioxygenase includes PcaH and PcaG, the exogenous dioxygenase includes LigA and LigB, the microbial cell is capable of growth utilizing at least one of a cellulose decomposition molecule or a lignin decomposition molecule, and the microbial cell is capable of producing 2-hydroxy-2H-pyran-4,6-dicarboxylic acid.
Original languageAmerican English
Patent number11,326,190 B2
Filing date10/05/22
StatePublished - 2022

NREL Publication Number

  • NREL/PT-2A00-82927

Keywords

  • amino acid sequence
  • endogenous dioxygenase
  • lignocellulose
  • nucleic acid sequence

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