Conversion of Corn Stover Hydrolysates to Acids: Comparison Between Clostridium carboxidivorans P7 and Microbial Communities Developed from Lake Sediment and an Anaerobic Digester: comparison between Clostridium carboxidivorans P7 and microbial communities developed from lake sediment and an anaerobic digester

Xiaowen Chen, Chunjie Xia, Aditi Kumar, Yanna Liang, Melvin Tucker

Research output: Contribution to journalArticlepeer-review

3 Scopus Citations

Abstract

Anaerobic fermentation is an environmentally sustainable technology for converting a variety of feedstocks to biofuels and bioproducts. Considering the complex nature of lignocellulosic hydrolysates, we aimed to investigate product formation from corn stover hydrolysates by using microbial communities under anaerobic conditions. A community developed from lake sediment was able to produce lactic acid from only glucose in the raw or overlimed hydrolysates. Another community from an anaerobic digester, however, was capable of using all hexose and pentose sugars in the raw and undetoxified hydrolysates and released lactic acid at 26.76 g/L. A pure acetogen, Clostridium carboxidivorans P7, was able to grow on the raw and overlimed hydrolysates, too. But the consumption of sugars was minimal and the total released acid concentrations were less than 2 g/L. Next generation sequencing of the enriched community derived from the anaerobic digester revealed the presence of Lactobacillus strains. The predominant species were Lactobacillus parafarraginis (72.6%) and L. buchneri (13.4%). Product titer from using this enriched community can be further enhanced by cultivating at fed-batch or continuous fermentation modes. Results from this study widened the door for producing valuable products from lignocellulosic feedstocks through using mixed cultures.

Original languageAmerican English
Pages (from-to)169-178
Number of pages10
JournalBiomass Conversion and Biorefinery
Volume8
Issue number1
DOIs
StatePublished - 2018

Bibliographical note

Publisher Copyright:
© 2017, Springer-Verlag Berlin Heidelberg.

NREL Publication Number

  • NREL/JA-5100-71117

Keywords

  • Anaerobic fermentation
  • Clostridium carboxidivorans P7
  • Corn stover hydrolysate
  • Lactic acid
  • Microbial community

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