Correction: Adipic Acid Production from Lignin: Adipic acid production from lignin (Energy Environ. Sci. (2015) 8 (617–628) DOI: 10.1039/C4EE03230F)

Derek Vardon, Mary Ann Franden, Christopher Johnson, Eric Karp, Michael Guarnieri, Jeffrey Linger, Michael Salm, Timothy Strathmann, Gregg Beckham

Research output: Contribution to journalArticlepeer-review

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Abstract

We have discovered that pMFL22, the plasmid that was used to replace catRBC with the tac promoter to cause accumulation of muconic acid and drive constitutive expression of the catechol dioxygenase gene catA in Pseudomonas putida KT2440, contains a mutation. Consequently, this mutation is also present in the muconate production strain MFL30 (P. putida KT2440 DcatRBC::Ptac:catA) and strains derived from it. The mutation occurs just upstream of catA, likely in or near its ribosome binding site (Fig. 1). To determine if this mutation might affect expression and, thus, activity of CatA in our muconate production strains, we repaired this mutation in the muconate production strain CJ200 (P. putida KT2440 DcatRBC::Ptac:catA pcaHG::Ptac:aroY:ecdB:asbF), a strain derived from MFL30 that was further engineered to produce muconate from sugars as well as aromatic molecules by the introduction of genes encoding a protocatechuate decarboxylase, aroY, an accessory protein required to produce a prenylated FMN cofactor required by AroY, ecdB, and a 3-dehydroshikimate dehydratase, asbF. 1 The performance of CJ200 was compared to the repaired strain, GB221, by growing the strains in M9 minimal media supplemented with 10 mM glucose and 30 mM benzoate at 30 1C, shaking at 225 rpm, and feeding an additional 10 mM glucose after 12 h (Fig. 2). (Figure Presented).

Original languageAmerican English
Pages (from-to)3534-3535
Number of pages2
JournalEnergy and Environmental Science
Volume15
Issue number8
DOIs
StatePublished - 26 Jul 2022

Bibliographical note

See NREL/JA-5100-64057 for original article

NREL Publication Number

  • NREL/JA-5100-83851

Keywords

  • adipic acid
  • lignin

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