Corrigendum to "Engineering glucose metabolism for enhanced muconic acid production in Pseudomonas putida KT2440" [Metab. Eng. 59 (2020) 64-75]

Gayle Bentley, Niju Narayanan, Ramesh Jha, Davinia Salvachua, Joshua Elmore, George Peabody, Brenna Black, Kelsey Ramirez, Annette De Capite, William Michener, Allison Werner, Dawn Klingeman, Heidi Schindel, Robert Nelson, Lindsey Foust, Adam Guss, Taraka Dale, Christopher Johnson, Gregg Beckham

Research output: Contribution to journalArticlepeer-review

Abstract

The plasmid used to delete hexR, pGB022, in our Pseudomonas putida KT2440 strain engineered to produce cis,cis-muconic acid from glucose, GB062, inadvertently introduced two mutations: a point mutation in the zwf-1, encoding the glucose-6-phosphate 1-dehydrogenase, that results in a conservative, V157I replacement and a deletion of the last 8 bp of the glucose-6-phosphate 1-epimerase gene, yeaD, leading to a frame shift in this gene and, consequently, the replacement of the last two amino acids of the C-terminus (LS) with 56 amino acids (RGPWVCPWVGKTAGCVSSRLFARSSIVLSVRLTYHLSVTLVCNVVVFTTLSLENRV).
Original languageAmerican English
Pages (from-to)66-67
Number of pages2
JournalMetabolic Engineering
Volume72
DOIs
StatePublished - Jul 2022

Bibliographical note

See NREL/JA-2A00-75899 for original article

NREL Publication Number

  • NREL/JA-2A00-82313

Keywords

  • glucose metabolism
  • Pseudomonas putida

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