Corrigendum to "Engineering glucose metabolism for enhanced muconic acid production in Pseudomonas putida KT2440" [Metab. Eng. 59 (2020) 64-75]

Research output: Contribution to journalArticlepeer-review

Abstract

The plasmid used to delete hexR, pGB022, in our Pseudomonas putida KT2440 strain engineered to produce cis,cis-muconic acid from glucose, GB062, inadvertently introduced two mutations: a point mutation in the zwf-1, encoding the glucose-6-phosphate 1-dehydrogenase, that results in a conservative, V157I replacement and a deletion of the last 8 bp of the glucose-6-phosphate 1-epimerase gene, yeaD, leading to a frame shift in this gene and, consequently, the replacement of the last two amino acids of the C-terminus (LS) with 56 amino acids (RGPWVCPWVGKTAGCVSSRLFARSSIVLSVRLTYHLSVTLVCNVVVFTTLSLENRV).
Original languageAmerican English
Pages (from-to)66-67
Number of pages2
JournalMetabolic Engineering
Volume72
DOIs
StatePublished - Jul 2022

Bibliographical note

See NREL/JA-2A00-75899 for original article

NLR Publication Number

  • NREL/JA-2A00-82313

Keywords

  • glucose metabolism
  • Pseudomonas putida

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