CRISPR Enabled Trackable Genome Engineering for Isopropanol Production in Escherichia coli

Violeta Sanchez i Nogue; Gregg Beckham; Liya Liang; Rongming Liu; Andrew Garst; Thomas Lee; Ryan Gill

CRISPR Enabled Trackable Genome Engineering for Isopropanol Production in Escherichia coli

Violeta Sanchez i Nogue, Gregg Beckham, Liya Liang, Rongming Liu, Andrew Garst, Thomas Lee, Ryan Gill

Research output: Contribution to conference › Paper › peer-review

Abstract

Isopropanol is an important target molecule for sustainable production of fuels and chemicals. Increases in DNA synthesis and synthetic biology capabilities have resulted in the development of a range of new strategies for the more rapid design, construction, and testing of production strains. Here, we report on the use of such capabilities to construct and test 903 different variants of the isopropanol production pathway in Escherichia coli.

Original language	American English
Pages	123-124
Number of pages	2
State	Published - 2017
Event	Synthetic Biology Conference: Engineering, Evolution, and Design, SEED 2017 - Vancouver, Canada Duration: 20 Jun 2017 → 23 Jun 2017

Conference

Conference	Synthetic Biology Conference: Engineering, Evolution, and Design, SEED 2017
Country/Territory	Canada
City	Vancouver
Period	20/06/17 → 23/06/17

NREL Publication Number

NREL/CP-5100-71977

Keywords

CRISPR EnAbled Trackable genome Engineering
engineered Escherichia coli
isopropanol
proteomic analysis
RBS library

Cite this

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title = "CRISPR Enabled Trackable Genome Engineering for Isopropanol Production in Escherichia coli",

abstract = "Isopropanol is an important target molecule for sustainable production of fuels and chemicals. Increases in DNA synthesis and synthetic biology capabilities have resulted in the development of a range of new strategies for the more rapid design, construction, and testing of production strains. Here, we report on the use of such capabilities to construct and test 903 different variants of the isopropanol production pathway in Escherichia coli.",

keywords = "CRISPR EnAbled Trackable genome Engineering, engineered Escherichia coli, isopropanol, proteomic analysis, RBS library",

author = "{Sanchez i Nogue}, Violeta and Gregg Beckham and Liya Liang and Rongming Liu and Andrew Garst and Thomas Lee and Ryan Gill",

year = "2017",

language = "American English",

pages = "123--124",

note = "Synthetic Biology Conference: Engineering, Evolution, and Design, SEED 2017 ; Conference date: 20-06-2017 Through 23-06-2017",

}

TY - CONF

T1 - CRISPR Enabled Trackable Genome Engineering for Isopropanol Production in Escherichia coli

AU - Sanchez i Nogue, Violeta

AU - Beckham, Gregg

AU - Liang, Liya

AU - Liu, Rongming

AU - Garst, Andrew

AU - Lee, Thomas

AU - Gill, Ryan

PY - 2017

Y1 - 2017

N2 - Isopropanol is an important target molecule for sustainable production of fuels and chemicals. Increases in DNA synthesis and synthetic biology capabilities have resulted in the development of a range of new strategies for the more rapid design, construction, and testing of production strains. Here, we report on the use of such capabilities to construct and test 903 different variants of the isopropanol production pathway in Escherichia coli.

AB - Isopropanol is an important target molecule for sustainable production of fuels and chemicals. Increases in DNA synthesis and synthetic biology capabilities have resulted in the development of a range of new strategies for the more rapid design, construction, and testing of production strains. Here, we report on the use of such capabilities to construct and test 903 different variants of the isopropanol production pathway in Escherichia coli.

KW - CRISPR EnAbled Trackable genome Engineering

KW - engineered Escherichia coli

KW - isopropanol

KW - proteomic analysis

KW - RBS library

UR - http://www.scopus.com/inward/record.url?scp=85048662954&partnerID=8YFLogxK

M3 - Paper

AN - SCOPUS:85048662954

SP - 123

EP - 124

T2 - Synthetic Biology Conference: Engineering, Evolution, and Design, SEED 2017

Y2 - 20 June 2017 through 23 June 2017

ER -

CRISPR Enabled Trackable Genome Engineering for Isopropanol Production in Escherichia coli

Abstract

Conference

NREL Publication Number

Keywords

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Cite this