Crystal structure of glycoside hydrolase family 127 β-l- arabinofuranosidase from Bifidobacterium longum

Tasuku Ito, Kyo Saikawa, Seonah Kim, Kiyotaka Fujita, Akihiro Ishiwata, Sophon Kaeothip, Takatoshi Arakawa, Takayoshi Wakagi, Gregg T. Beckham, Yukishige Ito, Shinya Fushinobu

Research output: Contribution to journalArticlepeer-review

30 Scopus Citations

Abstract

Enzymes acting on β-linked arabinofuranosides have been unknown until recently, in spite of wide distribution of β-l-arabinofuranosyl oligosaccharides in plant cells. Recently, a β-l-arabinofuranosidase from the glycoside hydrolase family 127 (HypBA1) was discovered in the newly characterized degradation system of hydroxyproline-linked β-l- arabinooligosaccharides in the bacterium Bifidobacterium longum. Here, we report the crystal structure of HypBA1 in the ligand-free and β-l-arabinofuranose complex forms. The structure of HypBA1 consists of a catalytic barrel domain and two additional β-sandwich domains, with one β-sandwich domain involved in the formation of a dimer. Interestingly, there is an unprecedented metal-binding motif with Zn2+ coordinated by glutamate and three cysteines in the active site. The glutamate residue is located far from the anomeric carbon of the β-l-arabinofuranose ligand, but one cysteine residue is appropriately located for nucleophilic attack for glycosidic bond cleavage. The residues around the active site are highly conserved among GH127 members. Based on biochemical experiments and quantum mechanical calculations, a possible reaction mechanism involving cysteine as the nucleophile is proposed.

Original languageAmerican English
Pages (from-to)32-37
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume447
Issue number1
DOIs
StatePublished - 2014

NREL Publication Number

  • NREL/JA-5100-62188

Keywords

  • Crystal structure
  • DUF1680
  • Human gut bacteria
  • Plant polysaccharide
  • Quantum mechanical calculations
  • Retaining glycoside hydrolase

Fingerprint

Dive into the research topics of 'Crystal structure of glycoside hydrolase family 127 β-l- arabinofuranosidase from Bifidobacterium longum'. Together they form a unique fingerprint.

Cite this