D-Xylose Transport by Candida succiphila and Kluyveromyces marxianus

Boris U. Stambuk; Mary Ann Franden; Arjun Singh; Min Zhang

doi:10.1385/ABAB:106:1-3:255

D-Xylose Transport by Candida succiphila and Kluyveromyces marxianus

Boris U. Stambuk, Mary Ann Franden, Arjun Singh, Min Zhang

Biosciences Center

Research output: Contribution to journal › Article › peer-review

41 Scopus Citations

Abstract

The kinetics and regulation of D-xylose uptake were investigated in the efficient pentose fermentor Candida succiphiIa, and in Kluyveromyces marxianus, which assimilate but do not ferment pentose sugars. Active high-affinity (K_m ∼ 3.8 mM; V_max ∼ 15 nmol/[mg·min]) and putative facilitated diffusion low-affinity (K_m ∼ 140 mM; V_max ∼ 130 nmol/[mg·min]) transport activities were found in C. succiphila grown, respectively, on xylose or glucose. K. marxianus showed facilitated diffusion low-affinity (K_m ∼ 103 mM; V_max ∼ 190 nmol/[mg·min]) transport activity when grown on xylose under microaerobic conditions, and both a low-affinity and an active high-affinity (K_m ∼ 0.2 mM; V_max ∼ 10 nmol/[mg·min]) transport activity when grown on xylose under fully aerobic conditions.

Original language	American English
Pages (from-to)	255-263
Number of pages	9
Journal	Applied Biochemistry and Biotechnology - Part A Enzyme Engineering and Biotechnology
Volume	106
Issue number	1-3
DOIs	https://doi.org/10.1385/ABAB:106:1-3:255 https://doi.org/10.1385/ABAB:106:1-3:255
State	Published - 2003

Bibliographical note

Posted with Permission

NREL Publication Number

NREL/JA-510-32364

Keywords

Candida succiphila
D-Xylose
Fermentation
Kluyveromyces marxianus
Transport kinetics

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https://www.nrel.gov/docs/gen/fy03/32364.pdf

Cite this

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title = "D-Xylose Transport by Candida succiphila and Kluyveromyces marxianus",

abstract = "The kinetics and regulation of D-xylose uptake were investigated in the efficient pentose fermentor Candida succiphiIa, and in Kluyveromyces marxianus, which assimilate but do not ferment pentose sugars. Active high-affinity (Km ∼ 3.8 mM; Vmax ∼ 15 nmol/[mg·min]) and putative facilitated diffusion low-affinity (Km ∼ 140 mM; Vmax ∼ 130 nmol/[mg·min]) transport activities were found in C. succiphila grown, respectively, on xylose or glucose. K. marxianus showed facilitated diffusion low-affinity (Km ∼ 103 mM; Vmax ∼ 190 nmol/[mg·min]) transport activity when grown on xylose under microaerobic conditions, and both a low-affinity and an active high-affinity (Km ∼ 0.2 mM; Vmax ∼ 10 nmol/[mg·min]) transport activity when grown on xylose under fully aerobic conditions.",

keywords = "Candida succiphila, D-Xylose, Fermentation, Kluyveromyces marxianus, Transport kinetics",

author = "Stambuk, {Boris U.} and Franden, {Mary Ann} and Arjun Singh and Min Zhang",

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language = "American English",

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T1 - D-Xylose Transport by Candida succiphila and Kluyveromyces marxianus

AU - Stambuk, Boris U.

AU - Franden, Mary Ann

AU - Singh, Arjun

AU - Zhang, Min

N1 - Posted with Permission

PY - 2003

Y1 - 2003

N2 - The kinetics and regulation of D-xylose uptake were investigated in the efficient pentose fermentor Candida succiphiIa, and in Kluyveromyces marxianus, which assimilate but do not ferment pentose sugars. Active high-affinity (Km ∼ 3.8 mM; Vmax ∼ 15 nmol/[mg·min]) and putative facilitated diffusion low-affinity (Km ∼ 140 mM; Vmax ∼ 130 nmol/[mg·min]) transport activities were found in C. succiphila grown, respectively, on xylose or glucose. K. marxianus showed facilitated diffusion low-affinity (Km ∼ 103 mM; Vmax ∼ 190 nmol/[mg·min]) transport activity when grown on xylose under microaerobic conditions, and both a low-affinity and an active high-affinity (Km ∼ 0.2 mM; Vmax ∼ 10 nmol/[mg·min]) transport activity when grown on xylose under fully aerobic conditions.

AB - The kinetics and regulation of D-xylose uptake were investigated in the efficient pentose fermentor Candida succiphiIa, and in Kluyveromyces marxianus, which assimilate but do not ferment pentose sugars. Active high-affinity (Km ∼ 3.8 mM; Vmax ∼ 15 nmol/[mg·min]) and putative facilitated diffusion low-affinity (Km ∼ 140 mM; Vmax ∼ 130 nmol/[mg·min]) transport activities were found in C. succiphila grown, respectively, on xylose or glucose. K. marxianus showed facilitated diffusion low-affinity (Km ∼ 103 mM; Vmax ∼ 190 nmol/[mg·min]) transport activity when grown on xylose under microaerobic conditions, and both a low-affinity and an active high-affinity (Km ∼ 0.2 mM; Vmax ∼ 10 nmol/[mg·min]) transport activity when grown on xylose under fully aerobic conditions.

KW - Candida succiphila

KW - D-Xylose

KW - Fermentation

KW - Kluyveromyces marxianus

KW - Transport kinetics

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U2 - 10.1385/ABAB:106:1-3:255

DO - 10.1385/ABAB:106:1-3:255

M3 - Article

C2 - 12721449

AN - SCOPUS:0037740781

SN - 0273-2289

VL - 106

SP - 255

EP - 263

JO - Applied Biochemistry and Biotechnology - Part A Enzyme Engineering and Biotechnology

JF - Applied Biochemistry and Biotechnology - Part A Enzyme Engineering and Biotechnology

IS - 1-3

ER -

D-Xylose Transport by Candida succiphila and Kluyveromyces marxianus

Abstract

Bibliographical note

NREL Publication Number

Keywords

Access to Document

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