D-Xylose Transport by Candida succiphila and Kluyveromyces marxianus

Boris U. Stambuk, Mary Ann Franden, Arjun Singh, Min Zhang

Research output: Contribution to journalArticlepeer-review

40 Scopus Citations

Abstract

The kinetics and regulation of D-xylose uptake were investigated in the efficient pentose fermentor Candida succiphiIa, and in Kluyveromyces marxianus, which assimilate but do not ferment pentose sugars. Active high-affinity (Km ∼ 3.8 mM; Vmax ∼ 15 nmol/[mg·min]) and putative facilitated diffusion low-affinity (Km ∼ 140 mM; Vmax ∼ 130 nmol/[mg·min]) transport activities were found in C. succiphila grown, respectively, on xylose or glucose. K. marxianus showed facilitated diffusion low-affinity (Km ∼ 103 mM; Vmax ∼ 190 nmol/[mg·min]) transport activity when grown on xylose under microaerobic conditions, and both a low-affinity and an active high-affinity (Km ∼ 0.2 mM; Vmax ∼ 10 nmol/[mg·min]) transport activity when grown on xylose under fully aerobic conditions.

Original languageAmerican English
Pages (from-to)255-263
Number of pages9
JournalApplied Biochemistry and Biotechnology - Part A Enzyme Engineering and Biotechnology
Volume106
Issue number1-3
DOIs
StatePublished - 2003

Bibliographical note

Posted with Permission

NREL Publication Number

  • NREL/JA-510-32364

Keywords

  • Candida succiphila
  • D-Xylose
  • Fermentation
  • Kluyveromyces marxianus
  • Transport kinetics

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