Demonstration of Parallel Algal Processing: Production of Renewable Diesel Blendstock and a High-Value Chemical Intermediate

Eric Knoshaug, Nicholas Nagle, Jonathan Stickel, Tao Dong, Eric Karp, Jacob Kruger, David Brandner, Nicholas Rorrer, Earl Christensen, Philip Pienkos, Ali Mohagheghi, Lorenz Manker, Deborah Hyman

Research output: Contribution to journalArticlepeer-review

32 Scopus Citations


Co-production of high-value chemicals such as succinic acid from algal sugars is a promising route to enabling conversion of algal lipids to a renewable diesel blendstock. Biomass from the green alga Scenedesmus acutus was acid pretreated and the resulting slurry separated into its solid and liquor components using charged polyamide induced flocculation and vacuum filtration. Over the course of a subsequent 756 hours continuous fermentation of the algal liquor with Actinobacillus succinogenes 130Z, we achieved maximum productivity, process conversion yield, and titer of 1.1 g L-1 h-1, 0.7 g g-1 total sugars, and 30.5 g L-1 respectively. Succinic acid was recovered from fermentation media with a yield of 60% at 98.4% purity while lipids were recovered from the flocculated cake at 83% yield with subsequent conversion through deoxygenation and hydroisomerization to a renewable diesel blendstock. This work is a first-of-its-kind demonstration of a novel integrated conversion process for algal biomass to produce fuel and chemical products of sufficient quality to be blend-ready feedstocks for further processing.

Original languageAmerican English
Pages (from-to)457-468
Number of pages12
JournalGreen Chemistry
Issue number2
StatePublished - 2018

Bibliographical note

Publisher Copyright:
© 2018 The Royal Society of Chemistry.

NREL Publication Number

  • NREL/JA-5100-70765


  • algal biomass
  • feedstocks
  • integrated conversion porcess


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