TY - JOUR
T1 - Detailed Analysis of Modifications in Lignin After Treatment with Cultures Screened for Lignin Depolymerizing Agents
AU - Gidh, Aarti
AU - Talreja, Dinesh
AU - Vinzant, Todd B.
AU - Williford, Todd Clint
AU - Mikell, Alfred
PY - 2006
Y1 - 2006
N2 - Termites, beetles, and other arthropods can digest living and decaying wood plus other lignocellulosic plant litter. Microbial sources like other wood-eating insect guts and wastewater treatment sludge were screened for lignin depolymerization. Near infrared spectroscopy and atomic force microscopy (AFM) along with high-performance liquid chromatography (HPLC), were used to track changes in functional groups, size, shape, and molecular weight of lignin molecules during incubations. Odontotaenius disjunctus (Betsy beetle) guts dissected whole or separately as midgut, foregut, and hindgut, consumed corn stover but did not show lignin depolymerization. The sludge-treated lignin did show some reduction in molecular weight on the HPLC, particle size (350-650 nm initially to 135-220 nm by day 30) and particles per field on AFM. pH and the presence of nutrients had a substantial effect on the extent of depolymerization. Cultures in lignin and nutrients showed higher growth than cultures with lignin only. Colony characteristics within the beetle gut and the sludge were also evaluated.
AB - Termites, beetles, and other arthropods can digest living and decaying wood plus other lignocellulosic plant litter. Microbial sources like other wood-eating insect guts and wastewater treatment sludge were screened for lignin depolymerization. Near infrared spectroscopy and atomic force microscopy (AFM) along with high-performance liquid chromatography (HPLC), were used to track changes in functional groups, size, shape, and molecular weight of lignin molecules during incubations. Odontotaenius disjunctus (Betsy beetle) guts dissected whole or separately as midgut, foregut, and hindgut, consumed corn stover but did not show lignin depolymerization. The sludge-treated lignin did show some reduction in molecular weight on the HPLC, particle size (350-650 nm initially to 135-220 nm by day 30) and particles per field on AFM. pH and the presence of nutrients had a substantial effect on the extent of depolymerization. Cultures in lignin and nutrients showed higher growth than cultures with lignin only. Colony characteristics within the beetle gut and the sludge were also evaluated.
KW - AFM
KW - Beetles
KW - Depolymerization
KW - HPLC
KW - Lignin
KW - NIR
UR - http://www.scopus.com/inward/record.url?scp=33646873717&partnerID=8YFLogxK
U2 - 10.1385/ABAB:131:1:829
DO - 10.1385/ABAB:131:1:829
M3 - Article
C2 - 18563657
AN - SCOPUS:33646873717
SN - 0273-2289
VL - 131
SP - 829
EP - 843
JO - Applied Biochemistry and Biotechnology - Part A Enzyme Engineering and Biotechnology
JF - Applied Biochemistry and Biotechnology - Part A Enzyme Engineering and Biotechnology
IS - 1-3
ER -