Determination of Total Lipids as Fatty Acid Methyl Esters (FAME) by in situ Transesterification: Laboratory Analytical Procedure (LAP): Issue Date: December 2, 2013; Revision Date: December 29, 2015

Stefanie Van Wychen; Lieve Laurens; Kelsey Kinley

doi:10.2172/1118085

Determination of Total Lipids as Fatty Acid Methyl Esters (FAME) by in situ Transesterification: Laboratory Analytical Procedure (LAP): Issue Date: December 2, 2013; Revision Date: December 29, 2015

Stefanie Van Wychen, Lieve Laurens, Kelsey Kinley

Research output: NREL › Technical Report

Abstract

This procedure is based on a whole biomass transesterification of lipids to fatty acid methyl esters to represent an accurate reflection of the potential of microalgal biofuels. Lipids are present in many forms and play various roles within an algal cell, from cell membrane phospholipids to energy stored as triacylglycerols.

Original language	American English
Number of pages	16
DOIs	https://doi.org/10.2172/1118085
State	Published - 2015

NREL Publication Number

NREL/TP-5100-60958

Keywords

algae
biomass
FAME
fatty acid methyl esters
laboratory analytical procedures (LAP)
LAPs
microalgal biofuels
total lipids
transesterification

Access to Document

10.2172/1118085

https://www.nrel.gov/docs/fy16osti/60958.pdf

Cite this

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title = "Determination of Total Lipids as Fatty Acid Methyl Esters (FAME) by in situ Transesterification: Laboratory Analytical Procedure (LAP): Issue Date: December 2, 2013; Revision Date: December 29, 2015",

abstract = "This procedure is based on a whole biomass transesterification of lipids to fatty acid methyl esters to represent an accurate reflection of the potential of microalgal biofuels. Lipids are present in many forms and play various roles within an algal cell, from cell membrane phospholipids to energy stored as triacylglycerols.",

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AU - Laurens, Lieve

AU - Kinley, Kelsey

PY - 2015

Y1 - 2015

N2 - This procedure is based on a whole biomass transesterification of lipids to fatty acid methyl esters to represent an accurate reflection of the potential of microalgal biofuels. Lipids are present in many forms and play various roles within an algal cell, from cell membrane phospholipids to energy stored as triacylglycerols.

AB - This procedure is based on a whole biomass transesterification of lipids to fatty acid methyl esters to represent an accurate reflection of the potential of microalgal biofuels. Lipids are present in many forms and play various roles within an algal cell, from cell membrane phospholipids to energy stored as triacylglycerols.

KW - algae

KW - biomass

KW - FAME

KW - fatty acid methyl esters

KW - laboratory analytical procedures (LAP)

KW - LAPs

KW - microalgal biofuels

KW - total lipids

KW - transesterification

U2 - 10.2172/1118085

DO - 10.2172/1118085

M3 - Technical Report

ER -

Determination of Total Lipids as Fatty Acid Methyl Esters (FAME) by in situ Transesterification: Laboratory Analytical Procedure (LAP): Issue Date: December 2, 2013; Revision Date: December 29, 2015

Abstract

NREL Publication Number

Keywords

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