Determination of Total Lipids as Fatty Acid Methyl Esters: Laboratory Analytical Procedure (LAP): Issue Date: December 2, 2013; Revision Date: December 29, 2015; Current Revision Date: November 1, 2023

Stefanie Van Wychen; Alicia Sowell; Kelsey Ramirez; Lieve Laurens

Determination of Total Lipids as Fatty Acid Methyl Esters: Laboratory Analytical Procedure (LAP): Issue Date: December 2, 2013; Revision Date: December 29, 2015; Current Revision Date: November 1, 2023

Stefanie Van Wychen, Alicia Sowell, Kelsey Ramirez, Lieve Laurens

Research output: NREL › Technical Report

Abstract

This Laboratory Analytical Procedure (LAP) covers the determination of total lipids expressed as fatty acid methyl esters (FAME). Results are reported as the percent FAME content based on the dry weight of the sample. The procedure is based on a whole biomass transesterification procedure of lipids to FAME, which eliminates the need for extraction and therefore is able to access all fatty acids in the biomass and represent an accurate reflection of the biofuels potential.

Original language	American English
Number of pages	17
State	Published - 2023

Bibliographical note

See NREL/TP-5100-60958 for previous version of this Laboratory Analytical Procedure

NREL Publication Number

NREL/TP-2700-87501

Keywords

algae
composition
fatty acids
laboratory analytical procedures (LAP)
lipids

Access to Document

https://www.nrel.gov/docs/fy24osti/87501.pdf

Cite this

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title = "Determination of Total Lipids as Fatty Acid Methyl Esters: Laboratory Analytical Procedure (LAP): Issue Date: December 2, 2013; Revision Date: December 29, 2015; Current Revision Date: November 1, 2023",

abstract = "This Laboratory Analytical Procedure (LAP) covers the determination of total lipids expressed as fatty acid methyl esters (FAME). Results are reported as the percent FAME content based on the dry weight of the sample. The procedure is based on a whole biomass transesterification procedure of lipids to FAME, which eliminates the need for extraction and therefore is able to access all fatty acids in the biomass and represent an accurate reflection of the biofuels potential.",

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T2 - Issue Date: December 2, 2013; Revision Date: December 29, 2015; Current Revision Date: November 1, 2023

AU - Van Wychen, Stefanie

AU - Sowell, Alicia

AU - Ramirez, Kelsey

AU - Laurens, Lieve

N1 - See NREL/TP-5100-60958 for previous version of this Laboratory Analytical Procedure

PY - 2023

Y1 - 2023

N2 - This Laboratory Analytical Procedure (LAP) covers the determination of total lipids expressed as fatty acid methyl esters (FAME). Results are reported as the percent FAME content based on the dry weight of the sample. The procedure is based on a whole biomass transesterification procedure of lipids to FAME, which eliminates the need for extraction and therefore is able to access all fatty acids in the biomass and represent an accurate reflection of the biofuels potential.

AB - This Laboratory Analytical Procedure (LAP) covers the determination of total lipids expressed as fatty acid methyl esters (FAME). Results are reported as the percent FAME content based on the dry weight of the sample. The procedure is based on a whole biomass transesterification procedure of lipids to FAME, which eliminates the need for extraction and therefore is able to access all fatty acids in the biomass and represent an accurate reflection of the biofuels potential.

KW - algae

KW - composition

KW - fatty acids

KW - laboratory analytical procedures (LAP)

KW - lipids

M3 - Technical Report

ER -

Determination of Total Lipids as Fatty Acid Methyl Esters: Laboratory Analytical Procedure (LAP): Issue Date: December 2, 2013; Revision Date: December 29, 2015; Current Revision Date: November 1, 2023

Abstract

Bibliographical note

NREL Publication Number

Keywords

Access to Document

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Cite this