Determination of Total, Organic, and Inorganic Carbon and Total Nitrogen in Biological Cultures and Liquid Fraction Process Samples: Laboratory Analytical Procedure (LAP): Issue Date: December 31, 2020; Revision Date: November 1, 2023

Alicia Sowell; Bonnie Panczak; Kaitlin Lesco; Stefanie Van Wychen; Lieve Laurens

Determination of Total, Organic, and Inorganic Carbon and Total Nitrogen in Biological Cultures and Liquid Fraction Process Samples: Laboratory Analytical Procedure (LAP): Issue Date: December 31, 2020; Revision Date: November 1, 2023

Alicia Sowell, Bonnie Panczak, Kaitlin Lesco, Stefanie Van Wychen, Lieve Laurens

Research output: NREL › Technical Report

Abstract

This Laboratory Analytical Procedure (LAP) describes the quantitative determination of total organic carbon, inorganic carbon in whole suspended biological samples and in the cell-free supernatant, by combustion and CO2 detection with a non-dispersive infrared (NDIR) detector. This allows for distinct reporting of soluble and insoluble organic and inorganic carbon. In addition, this procedure covers the quantitative determination of soluble nitrogen in the cell-free supernatant.

Original language	American English
Number of pages	20
State	Published - 2023

Bibliographical note

See NREL/TP-2700-78622 for previous version of this Laboratory Analytical Procedure

NREL Publication Number

NREL/TP-2700-87521

Keywords

algae
culture carbon efficiency
inorganic carbon
laboratory analytical procedures (LAP)
nutrient
organic carbon

Access to Document

https://www.nrel.gov/docs/fy24osti/87521.pdf

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title = "Determination of Total, Organic, and Inorganic Carbon and Total Nitrogen in Biological Cultures and Liquid Fraction Process Samples: Laboratory Analytical Procedure (LAP): Issue Date: December 31, 2020; Revision Date: November 1, 2023",

abstract = "This Laboratory Analytical Procedure (LAP) describes the quantitative determination of total organic carbon, inorganic carbon in whole suspended biological samples and in the cell-free supernatant, by combustion and CO2 detection with a non-dispersive infrared (NDIR) detector. This allows for distinct reporting of soluble and insoluble organic and inorganic carbon. In addition, this procedure covers the quantitative determination of soluble nitrogen in the cell-free supernatant.",

keywords = "algae, culture carbon efficiency, inorganic carbon, laboratory analytical procedures (LAP), nutrient, organic carbon",

author = "Alicia Sowell and Bonnie Panczak and Kaitlin Lesco and {Van Wychen}, Stefanie and Lieve Laurens",

note = "See NREL/TP-2700-78622 for previous version of this Laboratory Analytical Procedure",

year = "2023",

language = "American English",

type = "Other",

}

TY - GEN

T1 - Determination of Total, Organic, and Inorganic Carbon and Total Nitrogen in Biological Cultures and Liquid Fraction Process Samples: Laboratory Analytical Procedure (LAP)

T2 - Issue Date: December 31, 2020; Revision Date: November 1, 2023

AU - Sowell, Alicia

AU - Panczak, Bonnie

AU - Lesco, Kaitlin

AU - Van Wychen, Stefanie

AU - Laurens, Lieve

N1 - See NREL/TP-2700-78622 for previous version of this Laboratory Analytical Procedure

PY - 2023

Y1 - 2023

N2 - This Laboratory Analytical Procedure (LAP) describes the quantitative determination of total organic carbon, inorganic carbon in whole suspended biological samples and in the cell-free supernatant, by combustion and CO2 detection with a non-dispersive infrared (NDIR) detector. This allows for distinct reporting of soluble and insoluble organic and inorganic carbon. In addition, this procedure covers the quantitative determination of soluble nitrogen in the cell-free supernatant.

AB - This Laboratory Analytical Procedure (LAP) describes the quantitative determination of total organic carbon, inorganic carbon in whole suspended biological samples and in the cell-free supernatant, by combustion and CO2 detection with a non-dispersive infrared (NDIR) detector. This allows for distinct reporting of soluble and insoluble organic and inorganic carbon. In addition, this procedure covers the quantitative determination of soluble nitrogen in the cell-free supernatant.

KW - algae

KW - culture carbon efficiency

KW - inorganic carbon

KW - laboratory analytical procedures (LAP)

KW - nutrient

KW - organic carbon

M3 - Technical Report

ER -