Development and Validation of a Kinetic Model for Enzymatic Saccharification of Lignocellulosic Biomass

Kiran L. Kadam, Eric C. Rydholm, James D. McMillan

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252 Scopus Citations

Abstract

A multireaction kinetic model was developed for closed-system enzymatic hydrolysis of lignocellulosic biomass such as corn stover. Three hydrolysis reactions were modeled, two heterogeneous reactions for cellulose breakdown to cellobiose and glucose and one homogeneous reaction for hydrolyzing cellobiose to glucose. Cellulase adsorption onto pretreated lignocellulose was modeled via a Langmuir-type isotherm. The sugar products of cellulose hydrolysis, cellobiose and glucose, as well as xylose, the dominant sugar prevalent in most hemicellulose hydrolyzates, were assumed to competitively inhibit the enzymatic hydrolysis reactions. Model parameters were estimated from experimental data generated using dilute acid pretreated corn stover as the substrate. The model performed well in predicting cellulose hydrolysis trends at experimental conditions both inside and outside the design space used for parameter estimation and can be used for in silico process optimization.

Original languageAmerican English
Pages (from-to)698-705
Number of pages8
JournalBiotechnology Progress
Volume20
Issue number3
DOIs
StatePublished - 2004

NREL Publication Number

  • NREL/JA-510-33191

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