Development of Clostridium tyrobutyricum as a Microbial Cell Factory for the Production of Fuel and Chemical Intermediates from Lignocellulosic Feedstocks

Jeffrey Linger, Leah Ford, Kavita Ramnath, Michael Guarnieri

Research output: Contribution to journalArticlepeer-review

6 Scopus Citations

Abstract

Microbial conversion of lignocellulosic substrates to fuel and platform chemical intermediates offers a sustainable route to establish a viable bioeconomy. However, such approaches face a series of key technical, economic, and sustainability hurdles, including: incomplete substrate utilization, lignocellulosic hydrolysate, and/or end-product toxicity, inefficient product recovery, incompatible cultivation requirements, and insufficient productivity metrics. Development of a production host with native traits suitable for high productivity conversion of lignocellulosic substrates under process-relevant conditions offers a means to bypass the above-described hurdles and accelerate the development of microbial biocatalyst deployment. Clostridium tyrobutyricum, a native producer of short chain fatty acids, displays a series of characteristics that make it an ideal candidate for conversion of lignocellulosic substrates and thus represents a promising host for microbial production of diverse carboxylate-derived product suites. Herein, recent progress and future directions in the development of this bacterium as an industrial microbial cell factory, with emphases on the utilization of lignocellulosic substrates and metabolic engineering approaches, is reviewed.

Original languageAmerican English
Article number183
Number of pages15
JournalFrontiers in Energy Research
Volume8
DOIs
StatePublished - 2020

Bibliographical note

Publisher Copyright:
© Copyright © 2020 Linger, Ford, Ramnath and Guarnieri.

NREL Publication Number

  • NREL/JA-2700-77346

Keywords

  • biocatalysis
  • bioenergy
  • butyric acid
  • carboxylic acid
  • Clostridium tyrobutyricum ATCC 25755
  • metabolic engineering

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