TY - JOUR
T1 - Differential Detection of Genetic Loci Underlying Stem and Root Lignin Content in Populus
AU - Yin, Tongming
AU - Zhang, Xinye
AU - Gunter, Lee
AU - Priya, Ranjan
AU - Sykes, Robert
AU - Davis, Mark
AU - Wullschleger, Stan D.
AU - Tuskan, Gerald A.
PY - 2010
Y1 - 2010
N2 - In this study, we established a comprehensive genetic map with a large number of progeny from a three-generation hybrid Populus intercross, and phenotyped the lignin content, S/G ratio and 28 cell wall subcomponents both in stems and roots for the mapping individuals. Phenotypic analysis revealed that lignin content and syringyl-to-guaiacyl (S/G) ratio using pyrolysis molecular beam mass spectroscopy (pyMBMS) varied among mapping individuals. Phenotypic analysis revealed that stem lignin content is significantly higher than that in root and the quantified traits can be classified into four distinct groups, with strong correlations observed among components within organs. Altogether, 179 coordinating QTLs were detected, and they were co-localized into 49 genetic loci, 27 of which appear to be pleiotropic. Many of the detected genetic loci were detected differentially in stem and root. This is the first report of separate genetic loci controlling cell wall phenotypes above and below ground. These results suggest that it may be possible to modify lignin content and composition via breed and/or engineer as a means of simultaneously improving Populus for cellulosic ethanol production and carbon sequestration.
AB - In this study, we established a comprehensive genetic map with a large number of progeny from a three-generation hybrid Populus intercross, and phenotyped the lignin content, S/G ratio and 28 cell wall subcomponents both in stems and roots for the mapping individuals. Phenotypic analysis revealed that lignin content and syringyl-to-guaiacyl (S/G) ratio using pyrolysis molecular beam mass spectroscopy (pyMBMS) varied among mapping individuals. Phenotypic analysis revealed that stem lignin content is significantly higher than that in root and the quantified traits can be classified into four distinct groups, with strong correlations observed among components within organs. Altogether, 179 coordinating QTLs were detected, and they were co-localized into 49 genetic loci, 27 of which appear to be pleiotropic. Many of the detected genetic loci were detected differentially in stem and root. This is the first report of separate genetic loci controlling cell wall phenotypes above and below ground. These results suggest that it may be possible to modify lignin content and composition via breed and/or engineer as a means of simultaneously improving Populus for cellulosic ethanol production and carbon sequestration.
KW - carbon sequestration
KW - cellulosic ethanol production
KW - lignin content
KW - separate genetic loci
UR - http://www.scopus.com/inward/record.url?scp=78649506381&partnerID=8YFLogxK
U2 - 10.1371/journal.pone.0014021
DO - 10.1371/journal.pone.0014021
M3 - Article
C2 - 21151641
AN - SCOPUS:78649506381
SN - 1932-6203
VL - 5
JO - PLoS ONE
JF - PLoS ONE
IS - 11
M1 - e14021
ER -