Directing Clostridium ljungdahlii Fermentation Products via Hydrogen to Carbon Monoxide Ratio in Syngas

Joshua Jack; Jonathan Lo; Pin-Ching Maness; Zhiyong Ren

doi:10.1016/j.biombioe.2019.03.011

Directing Clostridium ljungdahlii Fermentation Products via Hydrogen to Carbon Monoxide Ratio in Syngas

Joshua Jack, Jonathan Lo, Pin-Ching Maness, Zhiyong Ren

Research output: Contribution to journal › Article › peer-review

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Abstract

Clostridium ljungdahlii was grown on syngas of varying composition to investigate how syngas blends used in fermentation can be tailored toward desired product formation. Experiments were conducted with liquid batch cultures exposed to the same volume of gas with varying ratios of hydrogen to carbon monoxide (H₂/CO). Formation of acetate was favored by higher concentrations of hydrogen in the headspace. The maximum acetate concentration of 35.21 ± 0.84 mM was obtained using an H₂/CO ratio of 2.0. Generation of more reduced metabolites was favored by greater carbon monoxide concentrations in the headspace. Maximum ethanol (7.53 ± 0.58 mM) and 2,3-Butanediol (5.20 ± 0.79 mM) concentrations were achieved under an H₂/CO ratio of 0.5. Linear relationships were obtained in that describe the effect of the syngas H₂/CO ratios on acetate, ethanol, and 2,3-BD formation, respectively.

Original language	American English
Pages (from-to)	95-101
Number of pages	7
Journal	Biomass and Bioenergy
Volume	124
DOIs	https://doi.org/10.1016/j.biombioe.2019.03.011 https://doi.org/10.1016/j.biombioe.2019.03.011
State	Published - May 2019

Bibliographical note

Publisher Copyright:
© 2019 Elsevier Ltd

NREL Publication Number

NREL/JA-2700-73838

Keywords

Clostridium ljungdahlii
Fermentation
H/CO ratio
Liquid fuel
Syngas

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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title = "Directing Clostridium ljungdahlii Fermentation Products via Hydrogen to Carbon Monoxide Ratio in Syngas",

abstract = "Clostridium ljungdahlii was grown on syngas of varying composition to investigate how syngas blends used in fermentation can be tailored toward desired product formation. Experiments were conducted with liquid batch cultures exposed to the same volume of gas with varying ratios of hydrogen to carbon monoxide (H2/CO). Formation of acetate was favored by higher concentrations of hydrogen in the headspace. The maximum acetate concentration of 35.21 ± 0.84 mM was obtained using an H2/CO ratio of 2.0. Generation of more reduced metabolites was favored by greater carbon monoxide concentrations in the headspace. Maximum ethanol (7.53 ± 0.58 mM) and 2,3-Butanediol (5.20 ± 0.79 mM) concentrations were achieved under an H2/CO ratio of 0.5. Linear relationships were obtained in that describe the effect of the syngas H2/CO ratios on acetate, ethanol, and 2,3-BD formation, respectively.",

keywords = "Clostridium ljungdahlii, Fermentation, H/CO ratio, Liquid fuel, Syngas",

author = "Joshua Jack and Jonathan Lo and Pin-Ching Maness and Zhiyong Ren",

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year = "2019",

month = may,

doi = "10.1016/j.biombioe.2019.03.011",

language = "American English",

volume = "124",

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T1 - Directing Clostridium ljungdahlii Fermentation Products via Hydrogen to Carbon Monoxide Ratio in Syngas

AU - Jack, Joshua

AU - Lo, Jonathan

AU - Maness, Pin-Ching

AU - Ren, Zhiyong

PY - 2019/5

Y1 - 2019/5

N2 - Clostridium ljungdahlii was grown on syngas of varying composition to investigate how syngas blends used in fermentation can be tailored toward desired product formation. Experiments were conducted with liquid batch cultures exposed to the same volume of gas with varying ratios of hydrogen to carbon monoxide (H2/CO). Formation of acetate was favored by higher concentrations of hydrogen in the headspace. The maximum acetate concentration of 35.21 ± 0.84 mM was obtained using an H2/CO ratio of 2.0. Generation of more reduced metabolites was favored by greater carbon monoxide concentrations in the headspace. Maximum ethanol (7.53 ± 0.58 mM) and 2,3-Butanediol (5.20 ± 0.79 mM) concentrations were achieved under an H2/CO ratio of 0.5. Linear relationships were obtained in that describe the effect of the syngas H2/CO ratios on acetate, ethanol, and 2,3-BD formation, respectively.

AB - Clostridium ljungdahlii was grown on syngas of varying composition to investigate how syngas blends used in fermentation can be tailored toward desired product formation. Experiments were conducted with liquid batch cultures exposed to the same volume of gas with varying ratios of hydrogen to carbon monoxide (H2/CO). Formation of acetate was favored by higher concentrations of hydrogen in the headspace. The maximum acetate concentration of 35.21 ± 0.84 mM was obtained using an H2/CO ratio of 2.0. Generation of more reduced metabolites was favored by greater carbon monoxide concentrations in the headspace. Maximum ethanol (7.53 ± 0.58 mM) and 2,3-Butanediol (5.20 ± 0.79 mM) concentrations were achieved under an H2/CO ratio of 0.5. Linear relationships were obtained in that describe the effect of the syngas H2/CO ratios on acetate, ethanol, and 2,3-BD formation, respectively.

KW - Clostridium ljungdahlii

KW - Fermentation

KW - H/CO ratio

KW - Liquid fuel

KW - Syngas

UR - http://www.scopus.com/inward/record.url?scp=85063734877&partnerID=8YFLogxK

U2 - 10.1016/j.biombioe.2019.03.011

DO - 10.1016/j.biombioe.2019.03.011

M3 - Article

AN - SCOPUS:85063734877

SN - 0961-9534

VL - 124

SP - 95

EP - 101

JO - Biomass and Bioenergy

JF - Biomass and Bioenergy

ER -

Directing Clostridium ljungdahlii Fermentation Products via Hydrogen to Carbon Monoxide Ratio in Syngas

Abstract

Bibliographical note

NREL Publication Number

Keywords

UN SDGs

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