DISCOVR strain pipeline screening – Part II: Winter and summer season areal productivities and biomass compositional shifts in climate-simulation photobioreactor cultures

Michael Huesemann, Song Gao, Scott Edmundson, Lieve Laurens, Stephanie Van Wychen, Nathan Beirne, Andrew Gutknecht, Robert Kruk, Kyle Pittman, Matthias Greer, Steffen Graham, Trenton Mueller

Research output: Contribution to journalArticlepeer-review

6 Scopus Citations

Abstract

Assessing the seasonal biomass productivity and compositional shift dynamics under simulated outdoor culture conditions of the top 21 algae strains selected during Tier I flask screening is an important step in the further prioritization of strains with regard to outdoor pond cultivation. These top 21 strains were subjected to Tier II testing in the PNNL Laboratory Environmental Algae Pond Simulator (LEAPS) photobioreactors, simulating light and temperature conditions of 20 cm deep outdoor ponds during the Arizona winter and summer season. All strains were grown in two consecutive nutrient-replete batch culture experiments at their particular optimal medium salinity to quantify their respective seasonal linear-phase areal biomass productivities. To determine biomass compositional shifts in response to nutrient-depletion, the LEAPS cultures were allowed to enter a 9-day nutrient depletion phase at the end of the second batch run. The following strains were evaluated in winter-season climate-simulated cultures and are listed in the order from highest (7.9 g m−2 day−1) to lowest (2.3 g m−2 day−1) areal N-replete biomass productivity: Monoraphidium minutum 26B-AM, Tetraselmis striata LANL1001, Chlorella vulgaris LRB AZ-1201, Micractinium reisseri NREL14-F2, Monoraphidium sp. MONOR1, Chlorella vulgaris NREL4-C12, Scenedesmus obliquus UTEX393, Scenedesmus acutus LRB-AP-0401, Nannochloropsis oceanica CCAP849/10, and Stichococcus minutus CCALA727. The following strains were evaluated in summer-season climate-simulated cultures and are listed in the order from highest (31.8 g m−2 day−1) to lowest (14.2 g m−2 day−1) areal N-replete biomass productivity: Picochlorum renovo NREL39-A8, Scenedesmus obliquus UTEX393, Porphyridium cruentum CCMP675, Picochlorum celeri TG2-WT-CSM/EMRE, Chlorella sorokiniana DOE1116, Stichococcus minor CCMP819, Picochlorum oklahomensis CCMP2329, Chlorella sorokiniana DOE1412 (UTEXB3016), Scenedesmus rubescens NREL46B-D3, Picochlorum soloecismus DOE101, Tetraselmis striata LANL1001, Scenedesmus obliquus DOE 0152.z, and Agmenellum quadruplicatum UTEX2268. All LEAPS cultures experienced a significant reduction in areal biomass productivity in response to nutrient-depletion, from 7 to 16 % in the winter season simulation and from 1 to 60 % in the summer season simulation. For 10 of the strains tested, the carbohydrate content more than doubled upon nutrient depletion, and for 9 strains, the lipid content increased by over 50 % of the initial content.

Original languageAmerican English
Article number102948
Number of pages8
JournalAlgal Research
Volume70
DOIs
StatePublished - 2023

Bibliographical note

Publisher Copyright:
© 2023 Elsevier B.V.

NREL Publication Number

  • NREL/JA-2700-82813

Keywords

  • Areal biomass productivity
  • Biomass compositional shift
  • Climate-simulation photobioreactors
  • Cold-season strain
  • Nitrogen depletion
  • Warm-season strain

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