Engineering B-ketoadipic Acid Production in Pseudomonas putida KT2440: Cooperative Research and Development Final Report, CRADA Number CRD-21-18379

Robert Baldwin; Gregg Beckham

doi:10.2172/3016090

Engineering B-ketoadipic Acid Production in Pseudomonas putida KT2440: Cooperative Research and Development Final Report, CRADA Number CRD-21-18379

BioEconomy and Sustainable Transportation

National Laboratory of the Rockies

Research output: NLR › Technical Report

Abstract

We propose a 2-year project to enable commercialization of an Agile Biofoundry (ABF) related, performance-advantaged bioproduct, B-ketoadipic acid (BKA), in an ABF-relevant, industrially-relevant host, Pseudomonas putida KT2440. Informed by techno-economic analysis (TEA), our BKA production goals are to enable a 40 g/L titer, 0.5 g/L/h productivity, and a 40% molar yield from hydrolysate sugars at the 1 kg scale. This material will be used to produce BKA-nylons.

Original language	American English
Number of pages	7
DOIs	https://doi.org/10.2172/3016090
State	Published - 2026

NLR Publication Number

NLR/TP-2A00-98487

Keywords

agile biofoundry (ABF)
B-ketoadipic acid (BKA)
CRADA
fluorescence biosensor (GFP)
high performance liquid chromatography (HPLC)
hydrolysate sugars
Pseudomonas putida KT2440

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10.2172/3016090

https://www.nlr.gov/docs/fy26osti/98487.pdf

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title = "Engineering B-ketoadipic Acid Production in Pseudomonas putida KT2440: Cooperative Research and Development Final Report, CRADA Number CRD-21-18379",

abstract = "We propose a 2-year project to enable commercialization of an Agile Biofoundry (ABF) related, performance-advantaged bioproduct, B-ketoadipic acid (BKA), in an ABF-relevant, industrially-relevant host, Pseudomonas putida KT2440. Informed by techno-economic analysis (TEA), our BKA production goals are to enable a 40 g/L titer, 0.5 g/L/h productivity, and a 40\% molar yield from hydrolysate sugars at the 1 kg scale. This material will be used to produce BKA-nylons.",

keywords = "agile biofoundry (ABF), B-ketoadipic acid (BKA), CRADA, fluorescence biosensor (GFP), high performance liquid chromatography (HPLC), hydrolysate sugars, Pseudomonas putida KT2440",

author = "Robert Baldwin and Gregg Beckham",

year = "2026",

doi = "10.2172/3016090",

language = "American English",

type = "Other",

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T1 - Engineering B-ketoadipic Acid Production in Pseudomonas putida KT2440: Cooperative Research and Development Final Report, CRADA Number CRD-21-18379

AU - Baldwin, Robert

AU - Beckham, Gregg

PY - 2026

Y1 - 2026

N2 - We propose a 2-year project to enable commercialization of an Agile Biofoundry (ABF) related, performance-advantaged bioproduct, B-ketoadipic acid (BKA), in an ABF-relevant, industrially-relevant host, Pseudomonas putida KT2440. Informed by techno-economic analysis (TEA), our BKA production goals are to enable a 40 g/L titer, 0.5 g/L/h productivity, and a 40% molar yield from hydrolysate sugars at the 1 kg scale. This material will be used to produce BKA-nylons.

AB - We propose a 2-year project to enable commercialization of an Agile Biofoundry (ABF) related, performance-advantaged bioproduct, B-ketoadipic acid (BKA), in an ABF-relevant, industrially-relevant host, Pseudomonas putida KT2440. Informed by techno-economic analysis (TEA), our BKA production goals are to enable a 40 g/L titer, 0.5 g/L/h productivity, and a 40% molar yield from hydrolysate sugars at the 1 kg scale. This material will be used to produce BKA-nylons.

KW - agile biofoundry (ABF)

KW - B-ketoadipic acid (BKA)

KW - CRADA

KW - fluorescence biosensor (GFP)

KW - high performance liquid chromatography (HPLC)

KW - hydrolysate sugars

KW - Pseudomonas putida KT2440

U2 - 10.2172/3016090

DO - 10.2172/3016090

M3 - Technical Report

ER -

Engineering B-ketoadipic Acid Production in Pseudomonas putida KT2440: Cooperative Research and Development Final Report, CRADA Number CRD-21-18379

Abstract

NLR Publication Number

Keywords

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