Enzymatic Conversion of Xylan Residues from Dilute Acid-Pretreated Corn Stover

Joseph Shekiro; Erik M. Kuhn; Michael J. Selig; Nicholas J. Nagle; Stephen R. Decker; Richard T. Elander

doi:10.1007/s12010-012-9786-5

Enzymatic Conversion of Xylan Residues from Dilute Acid-Pretreated Corn Stover

Joseph Shekiro, Erik M. Kuhn, Michael J. Selig, Nicholas J. Nagle, Stephen R. Decker, Richard T. Elander

National Renewable Energy Laboratory

Research output: Contribution to journal › Article › peer-review

26 Scopus Citations

Abstract

Enzymatic conversion of oligomeric xylose and insoluble xylan remaining after effective pretreatment offers significant potential to improve xylan-to-xylose yields while minimizing yields of degredation products and fermentation inhibitors. In this work, a commercial enzyme cocktail is demonstrated to convert up to 70 % of xylo-oligomers found in dilute acid-pretreated hydrolyzate liquor at varying levels of dilution when supplemented with accessory enzymes targeting common side chains. Commercial enzyme cocktails are also shown to convert roughly 80 % of insoluble xylan remaining after effective high-solids, dilute acid pretreatment.

Original language	American English
Pages (from-to)	421-433
Number of pages	13
Journal	Applied Biochemistry and Biotechnology - Part A Enzyme Engineering and Biotechnology
Volume	168
Issue number	2
DOIs	https://doi.org/10.1007/s12010-012-9786-5 https://doi.org/10.1007/s12010-012-9786-5
State	Published - 2012

NREL Publication Number

NREL/JA-5100-52347

Keywords

Cellulosic ethanol
Enzyme conversion
Lignocellulosic biomass
Xylan
Xylanase
Xylo-oligomer
Xylo-oligomer conversion
Xylose

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abstract = "Enzymatic conversion of oligomeric xylose and insoluble xylan remaining after effective pretreatment offers significant potential to improve xylan-to-xylose yields while minimizing yields of degredation products and fermentation inhibitors. In this work, a commercial enzyme cocktail is demonstrated to convert up to 70 % of xylo-oligomers found in dilute acid-pretreated hydrolyzate liquor at varying levels of dilution when supplemented with accessory enzymes targeting common side chains. Commercial enzyme cocktails are also shown to convert roughly 80 % of insoluble xylan remaining after effective high-solids, dilute acid pretreatment.",

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AU - Shekiro, Joseph

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AU - Selig, Michael J.

AU - Nagle, Nicholas J.

AU - Decker, Stephen R.

AU - Elander, Richard T.

PY - 2012

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AB - Enzymatic conversion of oligomeric xylose and insoluble xylan remaining after effective pretreatment offers significant potential to improve xylan-to-xylose yields while minimizing yields of degredation products and fermentation inhibitors. In this work, a commercial enzyme cocktail is demonstrated to convert up to 70 % of xylo-oligomers found in dilute acid-pretreated hydrolyzate liquor at varying levels of dilution when supplemented with accessory enzymes targeting common side chains. Commercial enzyme cocktails are also shown to convert roughly 80 % of insoluble xylan remaining after effective high-solids, dilute acid pretreatment.

KW - Cellulosic ethanol

KW - Enzyme conversion

KW - Lignocellulosic biomass

KW - Xylan

KW - Xylanase

KW - Xylo-oligomer

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Enzymatic Conversion of Xylan Residues from Dilute Acid-Pretreated Corn Stover

Abstract

NREL Publication Number

Keywords

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