Enzymatic Conversion of Xylan Residues from Dilute Acid-Pretreated Corn Stover

Joseph Shekiro, Erik M. Kuhn, Michael J. Selig, Nicholas J. Nagle, Stephen R. Decker, Richard T. Elander

Research output: Contribution to journalArticlepeer-review

25 Scopus Citations

Abstract

Enzymatic conversion of oligomeric xylose and insoluble xylan remaining after effective pretreatment offers significant potential to improve xylan-to-xylose yields while minimizing yields of degredation products and fermentation inhibitors. In this work, a commercial enzyme cocktail is demonstrated to convert up to 70 % of xylo-oligomers found in dilute acid-pretreated hydrolyzate liquor at varying levels of dilution when supplemented with accessory enzymes targeting common side chains. Commercial enzyme cocktails are also shown to convert roughly 80 % of insoluble xylan remaining after effective high-solids, dilute acid pretreatment.

Original languageAmerican English
Pages (from-to)421-433
Number of pages13
JournalApplied Biochemistry and Biotechnology - Part A Enzyme Engineering and Biotechnology
Volume168
Issue number2
DOIs
StatePublished - 2012

NREL Publication Number

  • NREL/JA-5100-52347

Keywords

  • Cellulosic ethanol
  • Enzyme conversion
  • Lignocellulosic biomass
  • Xylan
  • Xylanase
  • Xylo-oligomer
  • Xylo-oligomer conversion
  • Xylose

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